摘要目的 观察盐诱导激酶1(SIK1)对胰腺癌细胞增殖、迁移和侵袭的调控作用.方法 将SIK1模拟物转染胰腺癌细胞株HPAC、BxPC3和PANC-1,48 h后,实时定量聚合酶链反应(Real-time PCR)检测SIK1的表达,细胞计数试剂盒(CCK-8)检测SIK1对细胞增殖的作用,细胞划痕实验检测细胞的迁移能力,Transwell实验检测细胞的侵袭能力.结果 SIK1在胰腺癌HPAC、BxPC3和PANC-1细胞中相对表达低于正常胰腺细胞(0.16 ±0.06、0.16 ±0.03和0.19 ±0.03,P＜0.01);转染SIK1模拟物后,胰腺癌HPAC、BxPC3和PANC-1细胞内SIK1相对表达量明显升高(3.66±0.49、1.91±0.35和2.04±0.19,P＜0.01).过表达SIK1后,CCK-8实验结果显示,明显降低HPAC、BxPC3和PANC-1细胞的增殖(0.67±0.02比0.61±0.03,P＜0.01;0.79±0.03比0.70±0.02,P＜0.01;0.87 ±0.02比0.75±0.03,P＜0.01);划痕实验结果显示:明显抑制HPAC、BxPC3和PANC-1细胞迁移[(169.82±7.76)比(106.89±7.79) μm,P＜0.01;(106.95±6.29)比(62.79±9.55) μm,P＜0.01;(105.38±7.54)比(82.41±10.74) μm,P＜0.01];Transwell细胞侵袭实验显示:明显抑制HPAC、BxPC3和PANC-1细胞侵袭[(63.50±3.51)比(47.83±3.19)个,P＜0.01;(34.50±3.94)比(21.50±2.51)个,P＜0.01;(52.50±2.43)比(32.83±2.14)个,P＜0.01].结论 过表达SIK1后抑制HPAC、BxPC3和PANC-1细胞株增殖、迁移和侵袭的作用.

abstractsObjective To investigate the effect of salt-induced kinase 1 (SIK1) on the proliferation,migration and invasion of pancreatic cells.Methods SIK1-mimic was transfected into HPAC,BxPC3 and PANC-1 cell lines.The expression of SIK1 was detected by real-time quantitative polymerase chain reaction (Real-time PCR) after 48 h.Cell proliferation was measured by cell counting kit-8 (CCK-8).The migration ability was assayed by would healing test.Transwell invasion assay was used to measure cell invasion Results The expression of SIK1 in HPAC,BxPC3 and PANC-1 cells was significantly lower than that in normal pancreatic cells (0.16 ± 0.06,0.16 ± 0.03,0.19 ± 0.03,P ＜ 0.01).The expression of SIK1 in HPAC,BxPC3 and PANC-1 cells was significantly higher (3.66 ± 0.49,1.91 ±0.35 and 2.04 ±0.19,P ＜0.01) after transfection with SIK1 mimic.CCK-8 results showed that the proliferation of HPAC,BxPC3 and PANC-1 cells was significantly reduced (0.67 ±0.02 vs.0.61 ± 0.03,P＜0.01;0.79±0.03 vs.0.70 ±0.02,P＜0.01;0.87 ±0.02 vs.0.75 ±0.03,P＜0.01).The results of wound healing test showed that the migration of HPAC,BxPC3 and PANC-1 cells was inhibited [(169.82 ±7.76) vs.(106.89 ±7.79) μm,P＜0.01;(106.95 ±6.29) vs.(62.79 ±9.55) μm,P＜0.01;(105.38±7.54)vs.(82.41 ±10.74) μm,tPACN-1=4.287,P＜0.01].Transwell cell invasion assay showed significant inhibition of HPAC,BxPC3 and PANC-1 cell invasion [(63.50±3.51) vs.(47.83 ±3.19),P＜0.01;(34.50±3.94) vs.(21.50±2.51),P＜0.01;(52.50 ± 2.43) vs.(32.83 ± 2.14),P ＜ 0.01].Conclusion Overexpression of SIK1 inhibited the proliferation,migration and invasion of HPAC,BxPC3 and PANC-1 cell lines.