摘要目的 观察微小RNA(miRNA,miR)-542-3p在脑胶质瘤患者肿瘤组织中的表达及其对肿瘤细胞增殖、迁移的影响.方法 采用实时荧光定量聚合酶链反应(FQ-PCR)检测33例脑胶质瘤患者肿瘤组织及29例颅脑外伤患者正常脑组织中miR-542-3p的表达水平;合成miR-542-3pmimics及无关序列(miR-542-3p NC),并采用Lipofectamine 2000分别将miR-542-3p mimics和miR-542-3p NC转染至人胶质瘤U87细胞株;分别进行培养后,采用细胞计数试剂盒(CCK-8)检测细胞的增殖能力;划痕实验检测细胞的迁移能力;Transwell实验检测细胞的侵袭能力,评价miR-542-3p在胶质瘤细胞U87中的生物学功能.结果 微小RNA miR-542-3p在脑胶质瘤组织及正常脑组织中的相对表达量分别为0.74±0.06和1.06±0.09,胶质瘤组织中miR-542-3p的相对表达水平显著低于正常脑组织(t=3.03,P＜0.01).在人胶质瘤细胞株U87中分别转染miR-542-3pmimics和无关序列miR-542-3p NC后,转染miR-542-3mimics对U87细胞增殖能力无明显影响(t=0.236,P＞0.05).但U87细胞中转染miR-542-3p mimics后细胞的迁移能力和侵袭能力明显增强.结论 miR-542-3p在人胶质瘤细胞中表达水平减低,并在胶质瘤细胞中发挥抑制细胞迁移和侵袭.

abstractsObjective To evaluate microRNA (miRNA,miR)-542-3p expression in glioma and its effect on cell proliferation and migration.Methods The expression of miR-542-3p in tumor tissues of 33 patients with glioma and 29 normal brain tissues of patients with traumatic brain injury was examined by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect.Synthesize miR-542-3p mimics and unrelated sequences (miR-542-3p NC) were transfect into human glioma cells (U87) by Lipofectamine 2000,respectively.Cell proliferation,migration and invasion ability was examined by cell counting kit-8 (CCK-8),wound healing and Transwell assay respectively.Results The relative expression levels of miR-542-3 p in glioma and normal brain tissues were 0.74 ± 0.06 and 1.06 ± 0.09,respectively.The relative expression levels of miR-542-3p in glioma tissues were significandy lower than those in normal brain tissues (t =3.03,P ＜ 0.01).Mi-542-3p mimics and microRNAs-542-3p NC were transfected into human glioma cell line U87,and had no significant effect on the proliferation of U87 cells (t =0.236,P ＞ 0.05wever,after transfection of miR-542-3p mimics into U87 cells,the migration and invasion ability of cells increased significantly.Conclusion MiR-542-3p expression in human glioma cells is decreased,and inhibite cell migration and invasion in glioma cells.