摘要目的 观察微小RNA(miRNA,miR)-502-5p对结肠癌细胞DLD-1功能的影响.方法 选取结肠癌细胞DLD-1分别转染miR-502-5p mimic(5 μl)、control mimic(5μl)、miR-502-5p inhibitor (10μl)与control inhibitor(10μl).实时定量反转录聚合酶链反应(RT-qPCR)检测miR-502-5p的表达;细胞计数试剂盒(CCK-8)实验检测细胞的增殖能力;平板克隆实验检测细胞集落形成能力;Transwell实验检测细胞迁移与侵袭能力.结果 转染miR-502-5p mimic与NC mimic后,DLD-1细胞中miR-502-5p的相对表达量分别为319.31±33.16和1.08±0.22,DLD-1细胞中miR-502-5p提高显著(P＜0.05);转染miR-502-5p inhibitor与NC inhibitor后DLD-1细胞中miR-502-5p的相对表达量分别为0.28 ±0.04和0.99 ±0.11,DLD-1细胞中miR-502-5p显著降低(P＜0.05);转染miR-502-5p mimic后DLD-1细胞的增殖能力:miR-502-5p mimic实验组[(125±9)个]与controlmimic组[(112±14)个]之间差异无统计学意义(P＞0.05),而迁移[(621±90)个比(174±32)个]与侵袭[(531±45)个比(132 ±34)个]能力显著提高(P＜0.05);转染miR-502-5p inhibitor后DLD-1细胞的增殖能力:miR-502-5p inhibitor实验组[(118±22)个]与control inhibitor组[(100±15)个]之间差异无统计学意义(P＞0.05),而迁移[(133±50)个比(543±84)个]与侵袭[(119±45)个比(458 ±57)个]能力显著降低(P＜0.05).结论 miR-502-5p可促进DLD-1细胞的迁移与侵袭能力,但是不影响增殖功能.

abstractsObjective To investigate the effect of microRNA (miRNA,miR)-502-5p on colon cancer DLD-1 cells.Methods Colon cancer cell line DLD-1 was transfected by miR-502-5p mimic,control mimic,miR-502-5p inhibitor and control inhibitor respectively.The effect of miR-502-5p on the proliferation of DLD-1 cells was detected by cell counting kit-8 (CCK-8) assay.The effect of miR-502-5p on the colony forming ability of DLD-1 cells was detected by plate-cloning assay.Transwell assay was used to evaluate the effect of miR-502-5p on the migration and invasion of colon cells.Results The expression of miR-502-5p was significantly increased (319.31 ± 33.16 vs.1.08 ± 0.22) in DLD-1 cells after transfection with miR-502-5p mimic (P ＜ 0.05).miR-502-5p was significantly decreased (0.28 ±0.04 vs.0.99 ±0.11) in DLD-1 cells after transfection with miR-502-5p inhibitor (P ＜ 0.05).After transfection of miR-502-5p mimic,the proliferation and colony forming ability of DLD-1 cells [(125 ± 9) cells vs.(112 ± 14) cells] were not significantly changed (P ＞ 0.05),while migration [(621 ±90) cells vs.(174 ±32) cells] and invasion [(531 ±45) cells vs.(132 ±34) cells] ability were significantly increased (P ＜ 0.05).The proliferation and colony forming ability of DLD-1 cells [(118 ± 22) cells vs.(100 ± 15) cells] were not significantly changed after transfection of miR-502-5p inhibitor (P ＞ 0.05),but the migration [(133 ± 50) cells vs.(543 ± 84) cells] and invasion [(119 ± 45) cells vs.(458 ± 57) cells] ability were decreased significantly (P ＜ 0.05).Conclusion MiR-502-5p may promote the migration and invasion of DLD-1 cells,but has no effect on the proliferation function.