摘要目的:探讨人T细胞免疫球蛋白与黏蛋白1(Tim-1)在胶质母细胞瘤中的表达及对肿瘤细胞生物学功能的影响。方法:通过组织芯片免疫组织化学、蛋白质印迹法(Western blot)检测Tim-1在胶质母细胞瘤组织(西安百思达生物科技有限公司，GL805a、GL805c)及细胞株(购自中国科学院上海细胞研究所)中的表达；通过RNA干扰(RNAi)技术构建下调Tim-1表达的胶质母细胞瘤细胞株，采用 t检验比较Tim-1敲低组和Tim-1对照组细胞在增殖、侵袭、迁移上的差异。 结果:Tim-1在胶质母细胞瘤组织中的表达(142.769±37.552)高于正常脑组织(43.501±23.811)；在胶质母细胞瘤细胞株(U87、U251)中的表达高于人脑正常胶质细胞株(HEB)(HEB：0.339±0.021，U87：0.919±0.066， t＝21.910， P<0.01；U251：1.074±0.085， t＝12.559， P<0.01)；成功构建下调Tim-1的胶质母细胞瘤细胞株(sh-Tim-1-U87、sh-Tim-1-U251)；细胞计数试剂盒(CCK-8)结果显示下调Tim-1后肿瘤细胞活力显著降低(24 h sh-NC：0.379±0.059，sh-U87：0.223±0.046， t＝2.767， P<0.05；sh-U251：0.210±0.036， t＝5.637， P<0.01。48 h sh-NC：0.677±0.034，sh-U87：0.444±0.052， t＝4.968， P<0.01；sh-U251：0.441±0.056， t＝8.831， P<0.01。72 h sh-NC：0.801±0.052，sh-U87：0.579±0.060， t＝4.487， P<0.01；sh-U251：0.636±0.056， t＝8.991， P<0.01)；划痕实验结果表明下调Tim-1后肿瘤细胞迁移能力显著降低(sh-NC：74.667±4.510，sh-U87：44.333±4.509， t＝11.651， P<0.01；sh-U251：34.003±3.606， t＝18.401， P<0.01)；Transwell实验显示下调Tim-1后肿瘤细胞侵袭能力显著降低(sh-NC：202.000±10.149，sh-U87：108.333±7.506， t＝11.294， P<0.01；sh-U251：137.667±11.061， t＝12.763， P<0.01)。 结论:Tim-1在胶母细胞瘤组织和细胞株中高表达，下调Tim-1可有效抑制胶质母细胞瘤细胞的增殖、迁移和侵袭能力。

abstractsObjective:To investigate the expression of human T cell immunoglobulin and mucin 1 (Tim-1) in glioblastoma and its effect on the biological function of tumor cells.Methods:The expression of Tim-1 in glioblastoma tissues (Xi’an Best Biological Technology Co., LTD., GL805a, GL805c) and cell lines (purchased from Shanghai Institute of Cell Research, Chinese Academy of Sciences) was detected by immunohistochemistry and Western blot. Glioblastoma cell lines with down-regulated Tim-1 expression were constructed by RNA interference (RNAi), and t-test was used to compare the differences in cell proliferation, invasion and migration between the Tim-1 knockdown group and the Tim-1 control group. Results:The expression of Tim-1 in glioblastoma tissues (142.769±37.552) was higher than that in normal brain tissues (43.501±23.811). The expression of glioblastoma cell lines (U87, U251) was higher than that of normal human glioblastoma cell lines (HEB: 0.339±0.021, U87: 0.919±0.066, t=21.910, P<0.01; U251: 1.074±0.085, t=12.559, P<0.01); Glioblastoma cell lines (sh-Tim-1-U87, sh-Tim-1-U251) down-regulating Tim-1 were successfully constructed. Results of cell counting kit-8 (CCK-8) showed that tumor cell activity was significantly decreased after Tim-1 was down-regulated (24 h sh-NC: 0.379±0.059, sh-U87: 0.223±0.046, t=2.767, P<0.05; sh-U251: 0.210±0.036, t=5.637, P<0.01; 48 h sh-NC: 0.677±0.034, sh-U87: 0.444±0.052, t=4.968, P<0.01; sh-U251: 0.441±0.056, t=8.831, P<0.01. 72 h sh-NC: 0.801±0.052, sh-U87: 0.579±0.060, t=4.487, P<0.01; sh-U251: 0.636±0.056, t=8.991, P<0.01); The results of the scratch test showed that tumor cell migration ability was significantly reduced after down-regulation of Tim-1 (sh-NC: 74.667±4.510, sh-U87: 44.333±4.509, t=11.651, P<0.01. sh-U251: 34.003±3.606, t=18.401, P<0.01); Transwell assay showed that the invasion ability of tumor cells was significantly reduced after down-regulation of Tim-1 (sh-NC: 202.000±10.149, sh-U87: 108.333±7.506, t=11.294, P<0.01; sh-U251: 137.667±11.061, t=12.763, P<0.01). Conclusion:Tim-1 is highly expressed in glioblastoma tissues and cell lines, and down-regulation of Tim-1 can effectively inhibit the proliferation, migration and invasion of glioblastoma cells.