摘要目的:探讨组合磁场(CMF)治疗对骨腱界面损伤修复的影响。方法:2018年7月至2019年7月，将由中南大学实验动物学部提供的24只成年新西兰雄兔建立髌骨部分切除模型，采用随机数字表法分为2组(每组12只)：治疗组术后给予CMF治疗(30 min/d)，对照组予以假治疗(设备关机状态)。术后12周收获髌骨-髌腱复合体标本行组织学、影像学和生物力学检测。组间比较采用独立样本 t检验。 结果:组织学染色结果显示，CMF治疗能明显促进愈合界面骨、纤维软骨的形成和成熟。影像学检测发现治疗组面积、长度、骨矿化含量、骨密度和骨显微形态学参数等量化指标明显优于对照组。拉断实验显示，治疗组的横截面积、拉断负荷、极限强度、拉断能量等力学性能指标[(29.58±4.55) mm 2、(229.2±59.4) N、(8.27±1.21) MPa、(0.478±0.149) J]明显高于对照组[(21.25±3.33) mm 2、(154.2±40.9) N、(6.57±1.16) MPa、(0.268±0.140) J， t＝3.619、2.547、2.484、2.516， P<0.05]。 结论:CMF治疗能明显促进骨腱界面损伤后修复。

abstractsObjective:The purpose of this study was to investigate the effects of combined magnetic fields (CMF) on bone-tendon interface (BTI) healing.Methods:From July 2018 to July 2019, Twenty-four mature male New Zealand rabbits (Provided by the Department of Experimental Animals, Central South University) with partial patellectomy were used to establish a BTI injury model, and were divided randomly into CMF treatment group (30 min/d) or placebo control group in which the CMF apparatus was turned off, according to the random number table. Their patella-patella tendon complexes were harvested at postoperative week 12 for histological staining, imaging analysis and biomechanical test.The independent sample t test was used for statistical analysis of the quantitative data. Results:The histological staining demonstrated that the CMF treatment obviously promoted the formation and maturation of new-formed bone and fibrocartilage. The quantitative indexes of the new-formed bone, such as area, length, bone mineralization content, bone mineralization density and bone micromorphological parameters, were obviously better in CMF group. Biomechanical test results showed that the cross-sectional area, failure load, ultimate strength and energy at failure in CMF group [(29.58±4.55) mm 2, (229.2±59.4) N, (8.27±1.21) MPa, (0.478±0.149) J] were significantly higher than those of the control group [(21.25±3.33) mm 2, (154.2±40.9) N, (6.57±1.16) MPa, (0.268±0.140) J, t=3.619, 2.547, 2.484, 2.516, P<0.05]. Conclusion:CMF therapy accelerated the healing process of BTI injury.