摘要目的:探讨鼠双微体基因2(MDM2)和细胞分裂周期蛋白20(CDC20)在原发性肝癌(PLC)组织中的表达、及其与PLC临床病理特征的关系。方法:收集2016年3月至2021年9月临沂市人民医院和山东医学高等专科学校附属医院病理确诊的25例正常肝组织标本、41例肝硬化组织标本、81例PLC组织标本，采用免疫组织化学方法检测上述组织中MDM2和CDC20表达，采用 χ2检验分析两者的表达与PLC临床病理特征的关系。 结果:正常肝组织、肝硬化组织与PLC组织中MDM2阳性表达率分别为12.00%(3/25)、36.59%(15/41)、62.96%(51/81)，两两之间比较差异有统计学意义( χ2＝ 4.733、19.854、7.627， P<0.05)；MDM2表达水平与PLC肿瘤大小、癌细胞分化程度、TNM分期(TNM stage)和血管浸润明显相关( χ2＝5.006、7.836、5.871、4.669， P<0.05)。正常肝组织、肝硬化组织与PLC组织中CDC20阳性表达率分别为16.00%(4/25)、34.21%(19/41)、67.90%(55/81)，两两之间比较差异有统计学意义( χ2＝6.297、5.302、20.852， P<0.05)；CDC20表达水平与PLC血管浸润、肿瘤大小、TNM分期明显相关( χ2＝6.860、5.299、6.800， P<0.05)。 结论:MDM2和CDC20在PLC组织中表达上调，MDM2和CDC20表达可能与PLC的发生、发展、转移有关，MDM2和CDC20有助于预测PLC进展和预后。

abstractsObjective:To investigate the expression of murine double minute 2 (MDM2) and cell division cycle 20 (CDC20) in primary liver carcinoma (PLC) tissues, and explore the relationship between their expression and clinicopathlogical characteristics.Methods:From March 2016 to September 2021, 25 normal liver tissues, 41 liver cirrhosis tissues and 81 PLC tissues confirmed by pathology in Linyi People’s Hospital and the Affiliated Hospital of Shandong Medical College were collected, and the expression of MDM2 and CDC20 in the above tissues was detected by immunohistochemisry. Chi-square test was used to analyze the relationship between the expression of MDM2 and CDC20 and clinicopathological features of PLC.Results:The positive expression rates of MDM2 in normal liver tissues, liver cirrhosis tissues and PLC tissues were 12.00% (3/25), 36.59% (15/41) and 62.96% (51/81), respectively, and the differences were statistically significant ( χ2= 4.733, 19.854, 7.627, P<0.05). The MDM2 expression level was correlated with tumor size, differentiation degree, tumor node metastasis (TNM) stage and vascular invasion ( χ2=5.006, 7.836, 5.871, 4.669, P<0.05). The CDC20 positive expression rates in normal liver tissues, liver cirrhosis tissues and PLC tissues were 16.00% (4/25), 34.21% (19/41) and 67.90% (55/81) respectively, and the differences were statistically significant ( χ2=6.297, 5.302, 20.852, P<0.05). The expression level of CDC20 was correlated with vascular invasion, tumor size and TNM stage of PLC ( χ2=6.860, 5.299, 6.800, P<0.05). Conclusion:The expression of MDM2 and CDC20 is up-regulated in PLC tissues, which may be related to the occurrence, development and metastasis of PLC. MDM2 and CDC20 may be biological indicators to predict the progression and prognosis of PLC.