摘要目的:通过生物信息学的方法探究SRY盒相关基因7(SOX7)在结肠癌中的表达及和临床病理、预后的相关性。方法:由癌症基因组图谱(TCGA)内下载有RNA测序的结肠癌患者524例，整理患者生存时间、肿瘤大小、临床分期、生存状态、分化程度、淋巴结转移、性别以及年龄等病理资料，没有缺失值的患者为475例，其中40例为正常样本，435例为肿瘤样本。应用R软件的DESeq2程序包对TCGA数据库内的475例正常组织与肿瘤组织间显著差异表达基因(DEGs)进行分析。匹配TCGA数据库，经过基于基因表达水平值的交互式分析平台(GEPIA)在线分析，以箱形图(Box Plot)形式表现，得出SOX7基因的差异表达。选取结肠癌中SOX7表达的中位数值，把结肠癌患者分为表达低水平与高水平组，探究患者病理资料和SOX7基因表达的相关性。对高表达和低表达组患者生存率差异应用卡普兰-迈尔生存分析(Kaplan-Meier)，患者总生存率当作衡量的标准以绘制生存曲线。对SOX7基因的京都基因与基因组百科全书(KEGG)、基因集富集分析(GSEA)和基因本体(GO)路径富集分析。组间比较采用 t检验或 χ2检验。 结果:应用TCGA数据库对结肠癌正常组织与癌症组织的差异表达基因进行分析，得到6 531个稳定基因和2 310个下调基因、2 218个上调基因。在TCGA数据集内，通过对40对配对样本比较显示，与正常组织比较，结直肠癌组织内SOX7表达水平显著降低( χ2＝15.607， P<0.05)。SOX7表达水平在患者年龄、肿瘤大小、性别、TNM分期、神经侵犯、脉管侵犯方面比较差异无统计学意义( χ2＝1.669、0.276、3.108、0.055、0.630、1.906， P>0.05)；SOX7表达水平在患者淋巴结转移、分化程度、浸润深度方面比较差异有统计学意义( χ2＝10.918、8.948、33.642， P<0.05)。与SOX7高表达组比较，低表达组患者总体生存率显著降低( χ2＝12.325， P<0.05)。 结论:SOX7在结肠癌组织内为低表达，和患者的临床病理特征及预后明显相关。

abstractsObjective:To explore the expression of SRY related high mobility group box 7 (SOX7) in colon cancer and its correlation with clinicopathology and prognosis through bioinformatics, so as to provide reference for clinical diagnosis, treatment and prognosis improvement of colon cancer patients.Methods:A total of 524 cases of colon cancer patients with RNA sequencing downloaded from the cancer genome atlas (TCGA) were included, Organize pathological data such as patient survival time, tumor size, clinical stage, survival status, differentiation degree, lymph node metastasis, gender, and age. There were 475 patients without missing values, including 40 normal samples and 435 tumor samples. The DESeq2 package of R software was used to analyze 475 differentially expressed genes (DEGs) between normal and tumor tissues in the TCGA database. Kaplan Meier survival analysis was applied to compare the survival rates of patients in the high expression and low expression groups. The overall survival rate of patients was used as a measure to plot the survival curve. Kyoto encyclopedia of genes and genomes (KEGG), gene set enrichment analysis (GSEA), and gene ontology (GO) pathway enrichment analysis of SOX7 gene. The comparison between groups was conducted using t test or χ2 test. Results:Using the TCGA database to analyze differentially expressed genes between normal and cancer tissues of colon cancer, 6 531 stable genes, 2 310 down regulated genes, and 2 218 up regulated genes were identified. In the TCGA dataset, a comparison of 40 paired samples showed that the expression level of SOX7 in colorectal cancer tissues was significantly reduced compared to normal tissue( χ2=15.607, P<0.05). There was no statistically significant difference in the expression level of SOX7 among patients in terms of age, tumor size, gender, TNM stage, nerve invasion, and vascular invasion ( χ2=1.669, 0.276, 3.108, 0.055, 0.630, 1.906, P<0.05); The expression level of SOX7 showed statistically significant differences in lymph node metastasis, differentiation degree, and infiltration depth among patients ( χ2=10.918, 8.948, 33.642, P<0.05).compared with the high expression group of SOX7, the overall survival rate of patients in the low expression group was significantly reduced ( χ2=12.325, P<0.05). Conclusion:SOX7 is lowly expressed in colon cancer tissues and is significantly correlated with the clinical pathological characteristics and prognosis of patients.