摘要目的 研究白细胞介素(IL)1β是否促进入肾小球系膜细胞(HMC)摄取氧化低密度脂蛋白(Ox-LDL)以及对其植物血凝素样受体(LOX-1)表达的作用,探讨IL-1β影响脂质摄取的可能作用途径.方法 脂质化学染色、流式细胞计数观察IL-1β对HMC脂质摄取的影响,分析LOX-1受体的功能及IL-1β影响脂质摄取的可能作用途径.实时定量PCR和Western印迹方法检测IL-1β对LOX-1表达的影响.结果 油红"O"染色发现IL-1β增强HMC对Ox-LDL的摄取;流式细胞仪计数发现IL-1β呈剂量和时间依赖性促进HMC摄取荧光Dil标记的Ox-LDL(Dil-Ox-LDL),抗LOX-1抗体部分阻断IL-1β诱导的HMC对Ox-LDL的摄取,约为单纯IL-1β组的89.8%(P<0.05).5μg/L IL-1β刺激HMC,LOX-1 mRNA于6 h达高峰,为对照的6.87倍,IL-1β剂量依赖性促进LOX-1 mRNA的表达,当刺激HMC 12 h,LOX-1 mRNA于10μg/L组达高峰,为对照的6.57倍.IL-1β剂量和时间依赖性促进LOX-1蛋白的表达.5μg/L IL-1β刺激HMC,LOX-1蛋白表达于24 h达高峰,为对照的1.88倍.10μg/L组刺激细胞24 h达高峰,LOX-1蛋白为对照的2.57倍.结论 HMC基础状态下摄取Ox-LDL,IL-1β可能通过LOX-1途径增强对Ox-LDL摄取,促进细胞内脂质的摄取,加重细胞内脂质失调.

abstractsObjective To analysis if intedeukin-1β (IL-1β) can regulate human mesangial cells (HMC) to uptake oxidized low density lipoprotein (Ox-LDL) and if the effect of IL-lβ be changed through the lectin-like oxidized low-density lipoprotein receptor 1 (LOX-l)pathway. Methods The uptake of HMC to Ox-LDL stimulated by IL-1β was observed using Oil Red "O" and flow cytometry. The level of LOX-1 in HMC induced by IL-1β and Ox-LDL was examined using real-time PCR and Western blotting. Results Uptake of Ox-LDL and Dil-Ox-LDL by HMC was up-regulated upon stimulation with IL-1β in a dose- and time-dependent manner. Intracellular mean fluorescence density of Dil-Ox-LDL with LOX-1 blocker in IL-1β stimulation group was decreased compared to that without blocker. The peak level of LOX-1 mRNA reached after 6 h of stimulation and was as high as 6.87-fold of control. IL-1β could induce LOX-1 mRNA expression in a dose-dependent manner. Treated with 10 μg/L IL-1β for 12 h, the upregulation effect on LOX-1 mRNA was as high as 6.57-fold of control. IL-1β could induce LOX-1 protein expression in a time- and dose-dependent manner. The peak level of LOX-1 protein reached after 24 h of stimulation of 5 μg/L IL-1β and was as high as 1.88-fold of control. Treated with 10 μg/L IL-1β for 24 h, the up-regulation effect on LOX-1 protein reached peak and was as high as 2.57-fold of control. IL-1β could induce LOX-1 mRNA and protein expression in a dosedependent manner. Conclusion The expression of LOX-1 can be up-regulated by IL-1β in a dose-dependent manner and the enhanced uptake of HMC to Ox-LDL stimulated by IL-1β partly through the LOX-1 pathway, which means the dyslipidemia of HMC can be enhanced by inflammatory cytokines.