摘要目的 探讨糖尿病(DM)大鼠肺组织血管紧张素Ⅱ1型受体(AT1R)的变化及其对转化生子因子β1(TGF-β1)表达的影响.方法 高糖高脂饲料喂养联合小剂量链脲佐菌素(STZ)鼠尾静脉注射建立DM大鼠模型20只,随机数字表法分为DM组10只和氯沙坦干预组(LST组)10只,LST组予以氯沙坦30 mg·kg-1·d-1灌胃12周,设正常对照组(NC组)10只.免疫组织化学检测各组大鼠肺组织AT1R表达,实时定量聚合酶链反应(RT-PCR)、Western blotting检测比较各组肺组织AT1R、TGF-β1表达.采用单因素方差分析比较组间差异,两两比较采用LSD检验,组间比较采用单因素方差分析.结果 与NC组相比,DM组大鼠肺组织AT1R、TGF-β1表达显著升高(AT1R mRNA:0.04±0.03比0.29±0.15,t=5.252; AT1R蛋白:0.62±0.05比0.77±0.05,t=6.736;TGF-β1 mRNA:0.10±0.05比0.73±0.16,t=12.377;TGF-β1蛋白:0.47±0.05比0.70±0.05,t=10.66;均P＜0.05),LST组大鼠肺组织AT1R、TGF-β1表达显著低于DM组(AT1R mRNA:0.292±0.148比0.068±0.024,t=4.741;AT1R蛋白:0.77±0.05比0.63 ±0.04,t=7.396;TGF-β1 mRNA:0.74±0.16比0.19±0.09,t=9.563;TGF-β1蛋白:0.702±0.047比0.439±0.018,t=16.601;均P＜0.05).结论 DM大鼠肺组织可能存在局部肾素-血管紧张素系统组分的激活,促进了TGF-β1表达的增加,可能参与了肺细胞外基质的改变.

abstractsObjective In order to explore the change of pulmonary angiotensin Ⅱ type 1 receptor (AT1R) in diabetic rats and its effect on expression of transforming growth factor-β1(TGF-β1).Method Twenty diabetes mellitus (DM) model rats were established via chain of high sugar and high fat diet in combination with small dose of streptozotocin caudal vein injection.They were randomly divided into DM group (n=10),losartan (LST) group (n=10).Each rat of LST group was fed with 30 mg· kg 1 · d-1,LST for 12 weeks by gavage.Ten normal rats were established as normal control (NC) group.Immunohistochemistry was used to assay the expression of AT1R in lung of each group rats.RT-PCR and western blotting was performed to compare the expression of AT1R and TGF-β1 between different group.One-way analysis of variance was applied to compare data among groups.LSD anaysis was used for comparision between two groups.Results Compared with NC group,the expression of pulmonary AT1R and TGF-β1 in DM group rats increased significantly (AT1R mRNA:0.04±0.03 vs 0.29±0.15,t=5.252; AT1R protein:0.62±0.05 vc 0.77±0.05,t=6.736; TGF-β 1 mRNA:0.10±0.05 vs 0.73±0.16,t=12.377 ; TGF-β 1 protein:0.47±0.05 vs 0.70± 0.05,t=10.66; all above P＜ 0.05).The expression of pulmonary AT1R and TGF-β1 in LST group rats significantly decresed,compared with DM group (AT1R mRNA:0.292±0.148 vs 0.068±0.024,t=4.741;AT1R protein:0.77±0.05 vs 0.63±0.04,t=7.396;TGF-β1 mRNA:0.74±0.16 vs 0.19±0.09,t=9.563;TGF-β1 protein:0.702 ± 0.047 vs 0.439± 0.018,t=16.601; all above P＜0.05).Conclusion Part of the reninangiotensin system may be activated in lung tissue of DM rats,it promoted the increase of TGF-β1 expression and involved in the change of lung extracellular matrix.