摘要目的 探讨结直肠癌组织中肌动蛋白结合蛋白transgelin-2的表达及其与临床病理因素的关系,评价transgelin-2在结直肠癌预后判断中的价值.方法 应用组织芯片结合免疫组化方法,检测2002年9月至2004年4月120例手术切除的结直肠癌患者组织中transgelin-2的表达情况,男性74例,女性46例,年龄26～89岁,中位年龄64.5岁.采用x2检验其与临床病理因素的关系,Log-rank检测生存率差别,Cox比例风险模型分析多个变量对生存时间的影响.结果 transgelin-2在结直肠癌组织中的阳性表达率为69.2％.transgelin-2的表达与分化程度(x2=5.420)、淋巴结转移(x2＝45.577)、远处转移(x2=12.009)及TNM分期(x2=47.577)相关(P＜0.05).transelin-2阳性表达者术后生存时间(39±5)个月,阴性表达者为( 59±3)个月,Kaplan-Meier生存分析显示,transgelin-2表达与生存时间密切相关(P =0.003).Cox分析显示,远处转移(RR=8.318,95％ CI:4.119～16.790)、淋巴结转移(RR=2.794,95％ CI:1.246～6.263)和transgelin-2表达(RR=1.834,95％ CI:1.118～2.793)是影响结直肠癌患者预后的独立因素(P＜0.05).结论 肌动蛋白结合蛋白transgelin-2的表达水平与结直肠癌临床病理因素及预后相关,可能成为判断结直肠癌转移和预后的潜在标志物.

abstractsObjectives To investigate the relationship between the expression of transgelin-2 and the clinicopathological factors of colorectal carcinoma and evaluate the value of transgelin-2 in prognostic assessment of the colorectal cancer patients.Methods Using tissue microarray and immunohistochemical methods,we examined transgelin-2 of 120 colorectal cancer patients received surgical treatment from September 2002 to April 2004,including 74 male and 46 female,age from 26 to 89 years.Analyzed the relationship between transgelin-2 and both the clinicpathologcial features and prognosis of the colorectal cancer by using x2 test and Kaplan-Meier survival analysis.Cox proportion hazard regression analysis was used to study the independent prognostic factors.Results The positive rate of transgelin-2 expression was 69.2％ in colorectal carcinoma. The transgelin-2 expression correlated with differentiation degree ( x2 =5.420),Iymph nodes metastasis ( x2 =45.577 ),distant metastasis ( x2 =12.009 ),and TNM staging (x2 =47.577).The survival time was (39 ±5 ) months in patients with positive expression of the trangelin-2,while (59 ± 3 ) months in patients with negative expression.The patient's survival time was statistically correlated with the transgelin-2 expression (P =0.003 ).Distant metastasis (RR =8.318,95％CI:4.119-16.790),lymph nodes metastasis (RR =2.794,95％ CI:1.246-6.263) and transgelin-2 expression (RR =1.834,95％ CI:1.118-2.973) were independent prognostic factors in patients with colorectal cancer (P ＜ 0.05 ). Conclusions The expression of transgelin-2 is conelated withclinicopathological features and prognosis in colorectal cancer,may be the potential marker of metastasis and the prognosis of colorectal cancer patients.