摘要胰腺癌患者外周血循环肿瘤细胞(CTC)可能与肿瘤复发及转移相关,CTC检测及对其表型、基因型等特征进行分析,有助于胰腺癌的早期诊断与鉴别诊断、癌变预测、疾病进展监控及化疗敏感性评估等.CTC可利用免疫磁珠、膜滤过、微流控芯片等技术进行分选,但不同方法的样本处理速度、CTC回收效率、纯度、细胞活性等存在较大差异.目前CTC在胰腺癌诊治中的临床应用仍很有限,通过方法学的完善与规范、CTC基因测序及体外培养并建立体内移植瘤模型等,有望获取更多肿瘤生物学信息,指导个体化治疗.

abstractsCirculating tumor cells(CTC) disseminate from primary tumors by undergoing epithelial mesenchymal transition that allow their entry into the circulation to drive metastatic formation in pancreatic cancer patients.Technological advances in detection and characterization of CTC are conducive to the early diagnosis,differential diagnosis,monitoring disease progression and predicating the probability of canceration or the chemotherapeutic efficacy.Nowadays,detection methods of CTC can be based on immunomagnetic beads technique,cell filtration or microfluidic chips technology,but there are great differences in the sample throughput,CTC recovery rate,purity,and CTC viability among them.Owing to the dilemma in detection methods,the intrinsic relevance between the biological characteristics of CTC and clinical manifestations is still not exactly elucidated.By the improved methodology,next generation sequencing technology and exploring the technique for culturing CTC in vitro and establishing xenotransplanted tumor model in nude mice,more and more biological information will be revealed,and finally,individualized treatment is achieved.