摘要目的 观察电针(EA)对阿尔茨海默病(AD)大鼠额叶小神经胶质细胞、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的影响.方法 正常24月龄SD大鼠48只,随机分成正常组、假手术组、模型组和EA组,每组大鼠12只.在立体定位仪下向双侧Meynert核注射微量凝聚态A1β．40(1 μl,10μg/μl)制备AD动物模型,选取百会、太溪、足三里穴电针治疗,频率2 Hz,强度1 mA,留针15 min,每天1次,6 d为1个疗程,疗程间隔1 d,治疗2个疗程.免疫组化法观察额叶活化小神经胶质细胞、IL-1β、TNF-α的表达.结果 模型组额叶活化小神经胶质细胞、IL-1β、TNF-α的表达增加,经EA治疗后,EA组活化小神经胶质细胞、IL-1β、TNF-α的表达较模型组减少,且差异具有统计学意义(P<0.01).结论 EA能阻抑AD模型大鼠额叶炎性反应,调整机体的免疫状态.

abstractsObjeetive To observe the effect of electroacupuncture (EA) on microglia, interleukin-1β, and tumor necrosis factor-or (TNF-α) in the frontal lobes of rats modeling Alzheimer's disease (AD). Methods Forty-eight normal SD rats 24 months old were randomly divided into a normal group, a sham operated group, a model group and an EA group, with 12 rats in each group. The AD model was established by stereotaxic microinjection of agglutinated amyloid-beta protein (A1β-40, 1 μl, 10 μg/μl) into the rats' bilateral Meynert nucleus. The effects of EA applied to the rats' "Baihui" ( DU 20) "Taixi" ( KI 3 )" and "Zusanli" ( ST 36) acupoints were observed. The EA treatment was applied once a day for 6 days as a course. The next course of treatment was continued after one day's rest and the total treatment involved 2 courses. The activated mieroglia and the expression of IL-1β, and TNF-α in the frontal lobe were determined by immunohistochemistry method. Results The number of cells exhibiting microglia, IL-1β, or TNF-α in the frontal lobes of rats in the model group increased significantly, but after EA treat-ment, cells showing microglia, IL-1β, or TNF-α decreased remarkably. There were significant differences in compar-ison with the model group (P≤0. 01). Conclusion Electroacupuncture treatment can depress inflammatory reac-tion in the frontal lobe of AD rats and adjust the rats' immunologic state.