摘要目的 观察低频脉冲电磁场对糖尿病大鼠急性后肢缺血微循环血管再生的影响.方法 选取雄性SD大鼠60只,用60 mg/kg体重链脲菌素腹腔注射建立糖尿病大鼠模型后,制备大鼠急性后肢缺血模型.造模成功后分成实验组和对照组,每组30只,实验组术后第1天即给予低频脉冲电磁场治疗,每天治疗2 h;对照组除正常饮食外不予任何处理.于术后第1天及术后第7,14,28天,采用激光多普勒技术检测2组大鼠缺血右后肢肌肉血流,2组分别于术后第7,14,28天时每次随机处死10只大鼠,分离缺血后肢膝关节至踝关节肌肉,分别采用免疫荧光方法 检测大鼠内皮细胞抗原-1(RECA-1)表达,ELSIA方法 检测血管内皮生长因子(VEGF)和成纤维细胞生长因子-2(FGF-2)的表达,并采用Western blot方法 检测VEGF、FGF-2及各自受体VEGFR2、FGFR1的表达.结果 术后第14,28天,实验组缺血后肢血流量和RECA-1阳性数量均明显高于对照组(P<0.05).FGF-2及其受体在各个时间段实验组均高于对照组(P<0.05),而VEGF及其受体无明显差异(P>0.05).结论 低频脉冲电磁场可以促进糖尿病大鼠急性后肢缺血的血管新生,机制可能为可以通过刺激血管内皮细胞释放FGF-2.

abstractsObjective To observe the effects of low-frequency pulsed electromagnetic fields (LFPEMFs) on microcirculation angiogenesis in the hindlimbs of diabetic rats with acute ischemia. Methods Models of acute hindlimb ischemia were established in 60 male Sprague-Dawley diabetic rats. The diabetes model was established using 60 mg/kg intraperitoneal injections of streptozotocin (STZ). Fasting blood glucose levels were greater than 300 mg/dL. The rats were randomly divided into experimental and control groups. The rats in the experimental group were exposed to low-frequency pulsed electromagnetic fields for 2 hours each day, while the control group was not given any treatment. Laser-Doppler perfusion was used to measure blood flow in the ischemic hindlimb on days 0,7, 14 and 28 after the operation. The immunofluorescence of rat endothelial cell antigen-1 ( RECA-1) was used to evaluate the changes in angiogenesis. The levels of vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) were determined by both Western blotting and ELISA, and VEGFR2 and FGFR1 levels in the ischemic skeletal muscle were determined by Western blotting on days 7, 14 and 28 after the operation. Results The average perfusion ratio was significantly greater in the experimental group at days 14 and 28 compared with the control group. RECA-1 density in the tissues had increased significantly in the experimental group at the 14th and 28th day. The same was observed for FGF-2 and its receptor, but there was no significant difference for VEGF or its receptor in either group. Conclusions LFPMEFs can promote angiogenesis in acute hindlimb ischemia of diabetic rats by up-regulating FGF-2. This suggests that LFPMEFs may be useful for preventing and treating lower limb ischemia in diabetic humans.