摘要目的 观察发光二极管(LED)红光照射对大鼠骨髓间充质干细胞(MSCs)增殖的影响.方法 通过体外培养SD大鼠骨髓MSCs,将LED红光光源置于MSCs上方2 cm处,红光波长620 nm,测得光功率密度为6.67 mW/cm2,能量密度分别设定为0,0.5,1和2 J/cm2,其中0 J/cm2组作为对照组,其它组作为实验组.0.5 J/cm2组、1 J/cm2组和2 J/cm2组分别给予75 s,150 s和300 s LED红光照射,12 h后重复照射1次,共照射3次.于照射后48 h分别采用细胞计数试剂盒-8(CCK-8)检测各组细胞增殖活性,采用EdU细胞增殖试剂盒检测各组细胞DNA复制水平,采用流式细胞仪检测各组MSCs细胞周期变化情况.结果 经620 nm红光照射后,发现各实验组细胞增殖活性及DNA复制水平均较对照组明显增强(P<0.05),细胞生长周期均较对照组明显缩短(P<0.05),且上述指标变化幅度均以0.5 J/cm2组最显著.结论 低能量LED红光照射能增强SD大鼠骨髓MSCs细胞DNA复制活性,缩短细胞生长周期,从而促进MSCs体外增殖.

abstractsObjective To study the effect of red light on the proliferation of mesenchymal stem cells ( BMSCs) . Methods BMSCs were isolated from Sprague-Dawley rats and cultured in vitro. They were irradiated with red light delivered by a light-emitting diode placed 2 cm above the cell layer. The light had a wavelength of 620 nm and a maximum power of 1 W. The measured optical power density was 6.67 mW/cm2. The cells were ran-domly divided into a control group and three experimental groups. The control group was not irradiated, while the oth-er 3 groups were irradiated at 0. 5, 1 and 2 J/cm2 for 75 s, 150 s or 300 s, repeated twice with an interval of 12 hours. Forty-eight hours after the irradiation, cellular proliferation was evaluated using CCK-8 assays and DNA repli-cation was quantified with a Cell-Light EdU. Changes in the cell cycle parameters were detected using flow cytometry. Results After the irradiation, the proliferation and DNA replication of the experimental group were significantly strengthened, while the cell growth cycle was significantly shortened compared with the control group, especially in the group irradiated at 0.5 J/cm2 . Conclusion Low-energy red light irradiation can promote the proliferation of mesenchymal stem cells in vitro by enhancing DNA replication and shortening the cell growth cycle.