摘要本研究建立了致病性黄疸出血群钩体的基因库和分子克隆，获得了多个重组DNA克隆，制备了黄疸出血热群，波摩那群，哈勒焦Bovis和帕托克I等4种特异性不同的DNA重组探针，用于Southern印迹分析，能够识别和区分不同属、群乃至株的钩体。进一步测定和分析两个重组DNA的核苷酸序列，确定了其限制性谱和编码的氨基酸序列。进而制备了两对特异性不同的聚合酶链反应（PCR）寡核苷酸引物（B1 .2和B3.4），能够分别扩增全部致病性钩体和黄疸出血群内各型钩体超微量DNA。具有高度敏感性和特异性，可发展为钩体病早期诊断和流行病学分类、鉴定的有力工具。

abstractsThe first genomic bank of icterohaemorrhagiae, leptospira was set up in this Lab. and a series of recombinant DNA clones were collected. From these DNA clones,a number of DNA radio-active labelled probes were used for DNA hybridization in Southern blotting technique.Then,the DNA sequences were analyzed.The restriction map and open reading frames of the amino-acid coded by the DNA sequence were determined. Two pairs of oligonucleotide primers for PCR were synthesized and DNA of leptospires were detected by means of polymerase chain reaction (PCR) .