摘要　　目的制备鉴定去甲肾上腺素完全抗原为制备高灵敏度和特异性抗去甲肾上腺素（NE）单克隆抗体提供条件。方法分别用戊二醛法（GA）和碳化二亚胺法（EDC）在pH4．5和pH9．0条件下将NE与载体蛋白（ BSA、OVA）耦联制备完全抗原，采用紫外全波长扫描、SDS-PAGE和FeCl3显色反应3种方法进行鉴定；完全抗原腹腔注射小鼠并监测血清抗体效价。结果3种方法鉴定结果表明NE完全抗原制备成功，与pH4．5相比，在pH9．0条件下随着耦联时间的延长，NE的耦联比增加显著。间接ELISA结果显示，“检测抗原”和“免疫抗血清”配组测定时，当包被的“检测抗原”测定同种耦联方法制备的“免疫抗原”免疫小鼠后获得的“免疫抗血清”时，血清抗体滴度观测值高于与“检测抗原”制备方法不同的免疫小鼠抗血清，如：A组（BSA-NE-GA）测定C组抗血清（OVA-NE-GA）时测定结果高于A组测定G组抗血清（ OVA-NE-EDC）。结论用制备的NE完全抗原免疫小鼠成功产生了抗NE抗体。

abstracts Objective To construct and identify norepinephrine ( NE) complete antigen for the preparation of high sensitive and specific anti-NE monoclonal antibody .Methods Glutaraldehyde ( GA) and 1-Ethyl-3-(3-Dimethylaminopropyl ) carbodiimide ( EDC) were used to cross-link NE with carrier pro-teins (BSA, OVA) for NE complete antigen preparation under conditions of pH 4.5 or pH9.0.Three assays including UV scanning , SDS-PAGE and FeCl3 color reaction were performed for identification of NE com-plete antigen.Serum antibody titers were evaluated in mice model induced by intraperitoneal immunization with NE complete antigen .Results NE complete antigens were successfully prepared as indicated by the three identification assays .The coupling ratio was significantly increased in a time-depended manner under the condition of pH9.0 in comparison to that in the condition of pH 4.5.Indirect ELISA results showed that , when coating antigens and serum antibodies were prepared with the same cross -linking method , the serum antibody titers were significantly higher than those with different methods .Conclusion Anti-NE antibodies were successfully prepared by immunizing mice with NE complete antigens .