摘要目的 研究树突状细胞(dendritic cell,DC)经沙棘黄酮(Seabuckthorn flavones,SF)处理后形态、表型与基因表达谱的变化.方法 DC培养液中添加SF 200μg/ml,培养7 d后光镜观察形态学变化;流式细胞术检测DC表面标记;构建RNA文库进行数字基因表达谱(DGE)分析,筛选差异表达基因,分析富集的gene ontology(GO)以及Kyoto encyclopedia of genes and genomes(KEGG)信号通路.结果 SF处理后,流式结果显示DC显著上调表达HLA-DR、CD80、CD83、CD86;DGE筛选出差异表达基因355个,上调176个,下调179个,GO富集主要在调节免疫系统过程、免疫系统发育等生物学途径;信号通路分析表明,显著富集的途径与炎症、免疫系统和癌症等疾病密切相关.结论 SF能促进DC共刺激分子与促成熟分子的上调表达,并对CD11a、SLAMF6、LMCD1、TSC22D3和IKZF3等免疫相关基因的表达有调节作用.

abstractsObjective To study the changes in morphology , phenotypes and gene expression pro-files of dendritic cells (DCs) following treatment with Seabuckthorn flavones (SF).Methods DCs were treated with 200μg/ml of SF and then cultured for 7 days.Changes in the morphology of DCs were observed under light microscope .Flow cytometry was used to detect DC surface molecules .Total RNA was extracted to construct the library for digital gene expression profiling ( DGE ) .Differentially expressed genes were screened out and further analyzed by gene ontology ( GO) enrichment analysis and Kyoto encyclopedia of genes and genomes ( KEGG ) pathway enrichment analysis .Results Compared with control group , SF treatment significantly enhanced the expression of HLA-DR, CD80, CD83 and CD86 on DCs.A total of 355 differentially expressed genes were screened out by DGE , including 176 up-regulated genes and 179 down-regulated genes .GO enrichment was mainly involved in the regulation and development of the immune sys -tem and other biological processes .KEGG pathway analysis showed that the significantly enriched pathways were closely related to inflammation , the immune system, cancer and other diseases .Conclusion SF can promote the expression of DC co-stimulatory molecules and pro-mature molecules, and regulate the expres-sion of immunity-related genes such as CD11a, SLAMF6, LMCD1, TSC22D3 and IKZF3.