摘要目的 研究分泌白细胞介素35(IL-35)的新型效应性调节性T细胞(IL-35-Treg)在肠道免疫应答特别是肠炎反应过程中的调控作用.方法 利用IL-35报告小鼠,通过流式细胞术分析肠道固有层中IL-35-Treg细胞的比例和特征;使用1.5％DSS自由饮水构建小鼠炎症性肠病(IBD)模型,分析剔除IL-35-Treg细胞对小鼠体重、病理损伤和IFN-γ 分泌的影响.结果 发现肠道固有层中占有最大比例的IL-35-Treg细胞,该群细胞主要来源于胸腺发育的Treg细胞,并且高表达抑制性分子程序性死亡受体1(PD-1);剔除IL-35-Treg细胞后小鼠体内T细胞分泌大量的IFN-γ,加重了DSS诱导的肠炎发生.结论 IL-35-Treg在肠炎免疫应答中起到重要的调控作用.

abstractsObjective To evaluate the function of interleukin-35 (IL-35)-producing regulatory T cells (IL-35-Treg) in regulating intestinal inflammatory immune response. Methods The percentages and characteristics of IL-35-Treg in the intestinal lamina propria of transgenic mice expressing IL-35 were ana-lyzed by flow cytometry. A mouse model of inflammatory bowel disease ( IBD) was established by giving 1. 5％ DSS in drinking water. Influences of IL-35-Treg depletion on mouse weight, pathological injury and the secretion of IFN-γ were analyzed. Results IL-35-Treg were enriched in the intestinal lamina propria, and mainly derived from thymic Treg (tTreg). Intestinal IL-35-Treg expressed high levels of programmed death 1 (PD-1). Depletion of IL-35-Treg in mice with DSS-induced IBD resulted in an aggravation through up-regulating the expression of IFN-γ. Conclusion IL-35-Treg might play an important role in the regula-tion of intestinal inflammatory immune response.