摘要目的观察HepG2细胞因乙型肝炎病毒X抗原(HBx)转染导致的基因表达差异。方法①用逆转录病毒感染的方法建立表达乙型肝炎病毒X抗原(HepG2X)及氯霉素乙酰转移酶(HepG2Cat)的细胞模型。②用抑制差减杂交的方法做HepG2X和对照细胞(HepG2Cat)的cDNA文库差示筛选。③用32P随机引物标记法标记探针做Northern Blot杂交及地高辛缺口翻译标记法做原位分子杂交以筛选基因探针。结果经cDNA文库差减杂交及抑制性PCR扩增共得到10个cDNA探针，其中8个被HBx所启动，2个因HBx而表达下降。经检测，这些基因在HepG2X和对照细胞中的表达均有差异。在被HBx所抑制的2个差示表达基因中，1个与人的蛋白翻译起始因子同源，同时发现，此基因在正常组织中表达较强，而在肿瘤组织中表达被抑制。结论 HBx能够改变宿主细胞的基因表达，并且这些基因变化可能和肝癌发生有关。

abstractsObjective To evaluate differentially expressed genes between HepG2X and HepG2Cat cells. Methods 1. Recombinant retroviruses encoding the X antigen or bacterial chloramphenicol acetyltrans-ferase were constructed and used to infect HepG2-cells. 2. The differences in gene expression between HepG2X and HepG2Cat cells were then evaluated by suppression subtractive hybridization and PCR. 3. In Situ hybridization and northern blot analysis were carried out to screen these differentially expressed genes.Results PCR select cDNA subtraction generated 8 differentially expressed genes from HBx transfected HepG2 cells (turned on by HBx). All these probes distinguished HepG2X cells from HepG2Cat cells. Two bands (turned off by HBx) cloned from control cells, were detected in HepG2Cat cells but not in HepG2X. One dif-ferentially expressed gene C2, the human homology of hu-suil, encodes a translation initiation factor whose ex-pression was suppressed by X antigen in HepG2 cells. This gene was also found in most normal liver tissues,not in tumor tissues. Conclusion HBx can regulate the expression of genes whose products may be positive or negative regulators of cell growth. These changes may be in part of the mechanism contributes to hepatocellu-lar transformation.