摘要目的建立鼠慢性萎缩性胃炎(CAG)动物模型并探讨致萎缩因素。方法应用①60％酒精和20 mmol/L去氧胆酸钠(A因素)；②0.05％～0.1％氨水(B因素)；③0.05％吲哚美辛(C因素)按有重复的L8(27)正交设计对SD大鼠分三批进行制模刺激，三批刺激时间分别为3个月、6个月和9个月。实验到期后取出全胃，观察大鼠胃黏膜大体观及各项萎缩指标。结果应用A、B、C三因素(单因素或多因素)刺激6个月或9个月均出现典型的胃窦萎缩性胃炎征象，制模成功率达100％，且能维持形态稳定1个月以上。结论应用60％酒精和20 mmol/L去氧胆酸钠，0.05％～0.1％氨水，0.05％吲哚美辛对SD大鼠刺激6个月可成功地建立CAG模型，且稳定性良好。

abstractsObjective To establish a rat model of chronic atrophic gastritis and investigate the factors inducing gastric atrophy. Methods According to the repeated orthogonal design of Ls(27), 60% alcohol and 20 mmol/L sodium deoxycholate (served as factor A), 0.05% ～0.1% ammonia water (factor B) and 0.05% indomethacin (factor C) were given, alone or in combination, to rats in three experiments for 3 months, 6 months and 9 months, respectively. Then the rats were sacrificed and pathologic changes of the gastric mucosa were studied by gross appearence and microscopy. Results Typical appearance of CAG, which could maintain over one month, were found in all rats treated with factor A,B,C alone or in combination for 6or 9 months. Conclusion 60% alcohol, 20 mmol/L sodium deoxycholate, 0.05%～0.1% ammonia water and 0.05 % indomethacin given to SD rats for 6 months could establish animal model of CAG with a 100 %successful rate.