摘要目的 应用肝螺杆菌感染不同品系小鼠,观察肝螺杆菌的定植状况及病理特征,以期获得稳定的动物模型.方法 SPF级雄性BABL/c Cr、SCID/Cr、C57BL/6 Cr小鼠接种肝螺杆菌(H.hepaticus,Hh)标准菌株ATCC51450菌液0.2 ml(1×108 CUF/ml),连续3次,每次间隔48 h,对照组灌饲等量的PBS.于末次接种Hh后第4周、8周及16周处死小鼠,取出小鼠食管、胃、空肠、回肠、盲肠、结肠、肝脏及胰腺组织,行病理组织学检查、微需氧菌分离培养鉴定及Hh特异性16SrRNA基因扩增.结果 BALB/c Cr小鼠和SCID/ Cr小鼠接种Hh后4周、8周及16周盲肠定植率均为8/8,4周、8周及16周结肠定植率分别为(4/8、5/8、5/8)和(3/8、6/8、5/8),16周回肠及空肠定植率均为1/8,8周、16周肝脏定植率分别为(2/8、3/8)和(2/8、2/8);C57BL/6 Cr小鼠接种Hh后4周、8周16周盲肠定植率分别为1/8、2/8和2/8,8周、16周结肠定植率分别为1/8、2/8.Hh感染的BALB/c Cr和SCID/Cr小鼠肝脏、盲肠及结肠炎症改变较C57BL／6 Cr小鼠更为明显(P＜0.01),且随着感染时间的延长病理组织学评分逐渐增加(P值分别＜0.05和0.01),其中结肠及肝脏组织中Hh定植者的组织学评分明显高于无Hh定植者(P＜0.05),盲肠组织的病理组织学评分随着Hh定植密度的增加而增加(P＜0.05).结论 不同品系小鼠对Hh的易感性不同；BALB/cCr、SCID/Cr小鼠接种Hh后可获得较好的Hh感染模型.

abstractsObjective To obtain stable animal models and observe Helicobacter hepaticas (Hh)colonization and pathological claracteristics,through infecting different mice strains with Hh.Methods SPF-class male BABL/c Cr,SCID/Cr and C57BL/6 Cr mice were inoculated 0.2 ml Hh standard strain ATCC51450 bacterial suspension (1 × 108CUF/ml),inoculated for 3 times with 48 hours intervals,the control group was fed with the same volume of PBS.Mice were executed at 4 weeks,8weeks and 16 weeks since last Hh inoculation,and mice esophagus,stomach,jejunum,ileum,cecum,colon,liver and pancreas tissue were taken for histopathology examination,Micro-aerobic bacteria isolation,culture and identification and Hh specific 16S rRNA gene amplification.Results The colonization rates of Hh in cecum after inoculated in BALB/c Cr mice and SCID/Cr mice at 4 weeks,8 weeks and 16 weeks were all 8/8,colonization rates in colon at 4 weeks,8 weeks and 16 weeks were 4/8,5/8,5/8 and 3/8,6/8,5/8 respectively,colonization rates in ileum and jejunum at 16 weeks were 1/8,colonization rates in liver at 8 weeks and 16 weeks were 2/8,3/8 and 2/8,2/8respectively.The colonization rates of Hh in cecum after inoculated in C57BL/6 Cr mice at 4 weeks,8weeks and 16 weeks were 1/8,2/8 and 2/8 respectively,colonization rates in colon at 8 weeks and 16weeks were 1/8,2/8 respectively.Compared with C57BL/6 Cr mice,the inflammatory changes in liver,cecum and colon were more significant in Hh infected BALB/c Cr and SCID/Cr mice (P＜0.01),and histological scores gradually increased as infection time extended (P＜0.05,P＜ 0.01 ).The histological scores were significantly higher in those with colon and liver Hh bacterial colonization than those without Hh bacterial colonization (P＜0.05).The histopathological score of cecal tissue was positively correlated with the density of Hh colonization.Conclusion Different mice strains are with different susceptibility to Hh,and better Hh infection model can be obtained in Hh inoculated BALB/c Cr and SCID/Cr mice.