摘要目的 观察病毒性心肌炎(VMC)小鼠不同时间点白细胞介素17和γ干扰素双阳性细胞(IL-17 +/IFN-γ+细胞)的变化,探讨其在VMC发病中的作用.方法 雄性Balb/c小鼠腹腔注射柯萨奇病毒B3( CVB3)建立VMC小鼠模型(VMC组),对照组注射等量磷酸盐缓冲液,在注射的第0、1、2、3、4和6周苏木精-伊红染色观察心肌病理改变并计算心肌病理积分,流式细胞仪检测小鼠IL-17+/IFN-γ+细胞占CD4+T细胞的比例.结果 VMC组小鼠心肌组织病理积分于第1周升高(1.8±0.5),第2周达到最高(2.8±0.5),第3周开始逐渐降低,VMC组各时点与对照组相应时点比较差异均有统计学意义(P均＜0.05).VCM组小鼠各时点脾IL-17+/IFN-γ+细胞维持在低水平,与对照组相应时点比较差异均无统计学意义(P均＞0.05),Spearman相关性分析显示IL-17+/IFN-γ+细胞与心肌病理积分无显著相关(P＜0.05).结论 VMC小鼠中存在少量IL-17 +/IFN-γ+细胞,但可能与VMC的炎性过程无关.

abstractsObjective To observe the alteration of interleukin-17 and interferon-γ double positive cells(IL-17 +/IFN-γ cells)in mice with coxsackie virus B3(CVB3)induced acute viral myocarditis(VMC).Methods VMC was induced in male Balb/c mice by peritoneal injection of CVB3.Control mice received PBS injection.At 0,1,2,3,4 and 6 weeks after injection,pathological scores were determined on hematoxylin-eosin stained heart sections and flow cytometric analysis was performed to evaluate the percent of IL-17+/IFN-γ+ cells among CD4+ T cells.Results Compared to control mice,the pathological scores of VMC mice were higher on CVB3 infection week 1(1.8 ± 0.5),peaked on week 2(2.8 ± 0.5)and declined thereafter.However,the proportion of IL-17 +/IFN-γ+ cells remained steadily at a low level throughout the observation period and was similar between VMC and control mice.Conclusions Our data shows that IL-17 +/IFN-γ+ cells might not be involved in the inflammation process of CVB3 induced VMC mice model.