摘要目的 研究人重组粒细胞集落刺激因子(rhG-CSF)动员的健康供者外周血采集物(G-PB)和骨髓采集物(G-BM)中NK细胞亚群的异同.方法 2009年7月至9月在北京大学血液病研究所接受异基因造血干细胞移植(allo-HSCT)的28名亲缘供者,采用流式细胞术测定两种移植物中淋巴细胞、NK细胞及NK细胞中IFN-γ(NK1)、IL-13(NK2)、TGF-β和IL-10(NKr)四种细胞因子的分泌情况.结果 G-PB中淋巴细胞占有核细胞百分比显著高于G-BM(P＜0.01).G-BM中NK细胞、NK1、NK2、NKr占淋巴细胞百分比明显高于G-PB(P＜0.05),NK2、NKr占NK细胞的比例亦明显高于G-PB(P＜0.01),但NK1占NK细胞的比例在二者之间差异并无明显统计学意义.G-BM中IL-13与IFN-γ比值、TGF-β与IFN-γ比值、IL-10与IFN-γ比值亦明显高于G-PB(P值分别为0.010、0.002、0.000).结论 尽管G-BM与G-PB中NK1的比例相近,但G-BM中NK2及NKr的比例上调进一步解释了G-BM较G-PB具有更低的免疫反应性.

abstractsObjective To analyze the difference in NK cell subsets between recombination human granulocyte colony -stimulating factor (rhG-CSF) mobilized peripheral blood grafts (G-PB) and bone marrow grafts(G-BM) from healthy donors. Methods From July 2009 to September 2009, G-PB and G-BM from 28 related donors were collected to analyze lymphocytes, NK cells and NK cell secretion of interferon-γ(IFN-γ,NK1 ), interleukin-13 (IL-13, NK2 ), transforming growth factor-β (TGF-β) and interleukin-10 ( IL-l0,NKr) by flow cytometry. Results The percentage of lymphocytes in G-PB was significantly higher than that in G-BM(P＜0.01). The proportions of NK cells and NK1, NK2, NKr subsets among lymphocytes were significantly higher in G-BM than in G-PB(P＜0.05 ), and so were the percentage of NK2, NKr cells among NK cells (P＜0.01 ), but no significant difference in the percentage of NK1 cells among NK cells between G-PB and G-BM. The ratios of IL-13 and IFN-γ, of TGF-β and IFN-γ, or of IL-l0 and IFN-γ in G-BM were significantly higher than those in GPB ( P = 0. 010, 0. 002, or 0. 000, respectively). Conclusion The increased proportion of NK2 and NKr in G-BM might be helpful to explain the lower immunoreactivity of G-BM than that of G-PB, although the proportion of NK1 in G-BM and G-PB is similar.