摘要目的 观察复方血栓通胶囊对糖尿病大鼠视网膜微血管病变的干预效果.方法 正常雄性Wistar大鼠作为正常对照组(N组),链脲佐菌素(STZ)诱导Wistar大鼠糖尿病模型.将STZ诱导的糖尿病大鼠分为糖尿病对照组(DM组)和复方血栓通胶囊治疗组(FXST组).DM组未接受其他任何治疗;FXST组按900 mg/kg的剂量,采用经食道灌胃给药方法给予复方血栓通颗粒胶囊进行治疗,每日1次.第20周时到达实验终点.行视网膜消化铺片,显微镜下观察微血管改变,计算内皮细胞/周细胞(E/P)比值,图象系统计算血管相对面积.并行透射电子显微镜检查.观察毛细血管基底膜厚度及周细胞、内皮细胞超微结构变化.结果 视网膜消化铺片显示,20周时,DM组大鼠视网膜毛细血管纡曲.管径大小不一,出现节段性膨大、节段性管腔狭窄甚至闭塞.周细胞数目显著减少,E/P比值增高,血管相对面积增大,可见较多无细胞毛细血管、周细胞鬼影等.FXST组大鼠视网膜改变糖尿病对照组轻,E/P比值、血管相对面积均更接近于N组.透射电子显微镜检查显示,DM组大鼠毛细血管基底膜明显增厚,而FXST组相应的超微改变轻微.结论 复方血栓通胶囊能有效地防治糖尿病大鼠视网膜微血管改变.

abstractsObjective To observe the effect of Fufang XueShuanTong (FXST) on prevention for retinal microangiopathy of diabetic rats. Methods Take the normal male Wistar rats as normal control group; take the streptozotocin (STZ) Wistar rats as diabetic model group. And then the diabetic model group was divided into two groups : diabetic control group (without other treatment) and FXST treatment group (with FXST at dose 900 mg/kg, by the way of given medicine from esophagus to stomach, 1 time/ day, experimental period was 20 weeks). When all the animals had been raised for 20 weeks, not only retinal digesting preparations were used,, the endothelium/perieyte ratio (E/P ratio) and micro-vascular changes were observed by microscope, vascular relative area were measured by image system,but also the thickness of capillary basement membrane, the ultrastructural changes of endothelium and pericyte were observed by transmission electron microscope. Results On the 20th week, retinal digesting preparations showed that acellular capillaries, irregular vessel nets, segmental expansion, segmental stricture even occlusion, pericyte number decreased obviously, E/P ratio increased, vascular relative area increased and ghosts of pericytes etc in diabetic control group. Compared to diabetic control group, the retinal changes of FXST treatment group was lighter, the E/P ratio and vascular relative area were closer to normal control group. Transmission electron microscopy results showed that thickness of basement membrane was increased in DM group, vascular changes was light in FXST treated group. Conclusions FXST can prevent the changes of micrangium in diabetic rats effectively.