摘要目的 建立外源性金黄色葡萄球菌和大肠埃希杆菌混合细菌性眼内炎动物模型,为进一步开发治疗复杂件眼内炎的新型药物打基础.方法 新西兰白兔42只84只眼,随机分为混合细菌组、金黄色葡萄球菌组和大肠埃希杆菌组等3个实验和1个生理盐水对照组,每组21只眼.实验组分别玻璃体腔注射0.1 ml的2×104菌落形成单位(CFU)/ml的混合细菌悬液(含金黄色葡萄球菌和大肠埃希杆菌各103)、104CFU/ml的金黄色葡萄球菌悬液和104CFU/ml大肠埃希杆菌悬液,对照组注射0.1 ml无菌生理盐水.分别于注射后6、12、24、48、72 h和7、14 d行裂隙灯显微镜、检眼镜、眼部A/B超和视网膜电图(ERG)检查;同时各眼抽取玻璃体液0.1 ml做细菌鉴定和计数,后剖取眼球做组织病理学观察.结果 临床观察发现,3个实验组注射后均有不同程度的炎症反应,而总变化趋势趋于一致,混合细菌组在注射后12 h出现明显的前房渗出等炎症反应,较金黄色葡萄球菌组和大肠埃希杆菌组早,48～72 h最重,4～7 d炎症反应开始缓慢减轻,10～14 d炎症明显减轻,角膜出现新生血管,玻璃体呈灰白色混浊.细菌培养发现,混合细菌组6 h～14 d阳性率为100%;金黄色葡萄球菌组6～72 h阳性率为100%,7～14 d阳性率为0;大肠埃希杆菌组6 h～7 d阳性率为100%,14 d阳性率为67.67%;对照组6 h～14 d阳性率为0.ERG检查,注射后各实验组在48 h后b波波幅均消失,生理盐水组ERG-b波波幅下降小于30%.病理学检查,注射后各实验组表现为眼内各组织的炎细胞浸润.结论 通过兔眼玻璃体腔注射金黄色葡萄球菌和大肠埃希杆菌的方法成功建立了稳定的混合细菌所致的复杂性眼内炎模型.

abstractsObjective To establish rabbit models of mixture-infectious endophthalmitis induced byexogenous staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Methods A total of 84eyes of 42 New Zealand white albino rabbits were randomly divided into 4 groups.There were 21 eyes ineach group.Rabbit eyes in group 1,2,3 and 4 received an intravitreal injection of 0.1 ml of mix bacterium(2×104 CFU/ml,including 103 S.aureus and 103 E.coli),S.aureus(104 CFU/ml),E.coli(104CFU/ml),and sterilized saline respectively.The eyes were examined by slit-lamp microscopy,ophthalmoscopy,A/B scan,electroretinography(ERG)and bacterial culture of vitreous humors at thetimepoints of 6,12,24,48 and 72 hours,and 4,7,10,14 days after intravitreal injection.All eyeballswere then enucleated for histopathological examination.Results Various degrees of inflammatoryreactions were presented in the 3 experimental groups after the injection,and the development trend of thedisease was nearly the same.In group 1 active intraocular inflammation like anterior chamber exudates,started at 12 hours after injection(which was early than that in group 2 and 3),aggravated between 48and 72 hours,alleviated slowly from 4 to 7 days,and was obviously better after 10 to 14 days while thecorneal neovascularization and vitreous gray opacity begun to form.The bacterial culture was positive ingroup 1(100%,6 hours to 14 days after injection),group 2(100%,6 hours to 3 days after injection)and group 3(100% from 6 hours to 7 days,and 67.67% at 14 days after injection).It was negative for group2(7 to 14 days after injection)and group 4(6 hours to 14 days after injection).The amplitude of ERG bwave dissapeard in group 1 to 3,and decreased less than 30% in group 4 from the 48th hour afterinjection.Histopathological examination revealed that all intraocular structures infiltrated withinflammatory cells.Conclusion Complicated endophthalmitis rabbit models can be successfullyestablished by intravitreal injection with S.aureus and E.coli.