摘要目的 探讨骨髓来源细胞(BMCs)在脉络膜新生血管(CNV)生成中的作用.方法 将绿色荧光蛋白(GFP)转基因小鼠骨髓细胞移植给野生型C57BL/6J小鼠建立嵌合体,应用532 nm倍频激光诱发嵌合体小鼠(处理组)和野生型小鼠(对照组)CNV.利用脉络膜铺片观察由GFP阳性细胞形成的新生血管;免疫荧光染色观察CNV中GFP阳性细胞是否表达血管内皮生长因子(VEGF)和碱件成纤维细胞生长因子(bFGF).结果 激光光凝后第29天,脉络膜铺片显示CNV中有大量绿色的骨髓来源细胞,由其组成的血管面积约占CNV表面积的16.22%;免疫荧光染色结果 显示,CNV区域的一些BMCs表达VEGF和bFGF.结论 BMCs通过参与新生血管组成及分泌促血管生成因子的方式,可能在CNV发生过程中发挥着重要作用.

abstractsObjective To investigate the role of bone marrow-derived cells (EMC) plays in choroidal neovascularization (CNV).Methods Green fluorescent protein (C-FP) chimeric mice were built by transplanting BMC from GFP transgenic mice to adult wild type C57BL/6J mice.Retinal laser photocoagulation was used to induce CNV in the chimeric mice (treated group) and adult wild type mice (control group).Four weeks later,choroidal flatmount was prepared to detect GFP positive BMC expression in the CNV lesions,and immunofluorescence stain was used to determine the expression of vascular endothelia growth factor (VEGF) and basic fibroblast cell growth factor (bFGF).Results Twenty-nine days after photocoagulation lots of GFP positive BMC presented in the CNV area,which accounted approximate 16.22% of the total CNV area.Some BMC in the CNV area expressed VEGF and bFGF.Conclusions BMC may play an important role in CNV by forming new vessles and secreting angiogenic factors.