摘要目的 观察脉络膜黑色素瘤中血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)的表达,探讨两者在脉络膜黑色素瘤血管生成、肿瘤生长和浸润转移中的作用. 方法用免疫组织化学SP法检测58例脉络膜黑色素瘤石蜡组织标本中VEGF和PEDF蛋白的表达,用原位杂交法检测其中39例石蜡组织中PEDF mRNA的表达. 结果 58例脉络膜黑色素瘤石蜡组织中PEDF蛋白阳性表达率为22.4%,VEGF阳性表达率为74.1%;PEDF蛋白在巩膜导管未受侵犯组阳性率高于巩膜导管受侵犯组,差异有统计学意义(P<0.05);VEGF蛋白在巩膜导管未受侵犯组阳性表达率低于巩膜导管受侵犯组,差异有统计学意义(P<0.05).39例脉络膜黑色素瘤石蜡组织中PEDF mRNA阳性表达率为46.2%,巩膜导管未受侵犯组PEDF mRNA阳性率高于巩膜导管受侵犯组,其差异有统计学意义(P<0.05). 结论 VEGF和PEDF的失衡在脉络膜黑色素瘤血管发生、肿瘤的生长和浸润转移中起着重要的作用.

abstractsObjective To investigate the expression of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in choroidal melanoma. Methods The expression of VEGF and PEDF protein in fifty-eight cases of paraffin-embeded choroidal melanoma samples was measured by immunohistochemistry, the expression of PEDF mRNA in thirty-nine ehoroidal melanoma samples was assayed by in situ hybridization. Results PEDF protein was detected in 13/58 cases (22.4%) of choroidal melanoma, the positive rate in non-sclera-invasion group (12/38, 31.6%) was higher (P<0.05) than that in sclera-invasion group (1/20,5%). VEGF protein was detected in 43/58 cases (64%) of choroidal melanoma, the positive rate in non-sclera-invasion group (25/38, 65.8%) was lower (P<0.05) than that in sclera-invasion group (18/20,90%). The expression of PEDF mRNA was detected in 18/39 (46.2) cases, the positive rate in non-sclera-invasion group was higher (P<0.05) than that in sclera-invasion group. Conclusions Imbalanced expression of VEGF and PEDF in ehoroidal melanoma may play a key role in the angiogenesis, tumor progression and metastasis.