摘要目的 观察快速眼压波动对大鼠眼部血流动力学的影响。方法 建立快速眼压波动Sprague-Dawley大鼠模型。其眼压在1 s内由12 mm Hg(1 mm Hg=0.133 kPa)下降至0 mm Hg，2 min后于1 s内由0 mm Hg上升至12 mm Hg。采用裂隙灯图像分析系统拍摄大鼠眼压12 mm Hg及0 mm Hg时眼前节像，测量虹膜血管管径直径。眼压变化前，变化后5、10、20、60 s，采用彩色多普勒超声诊断仪检测大鼠视网膜中央动脉(CRA)和视网膜中央静脉(CRV)的血流动力学变化。测定峰值血流速度(PSV)、舒张期末流速( EDV)、平均血流速度(MV)、阻力指数(RI)、搏动指数(PI)和血管内径(VD)。结果 大鼠眼压为12 mm Hg时，其虹膜血管直径为(28.30±5.80) μm;大鼠眼压为0 mm Hg时，其虹膜血管直径为(59.80±6.40) μm;两者比较，差异有统计学意义（t=8.98，P＜0.01）。眼压快速下降时，大鼠虹膜血管、CRA和CRV的VD立即扩大；PSV、EDV和MV立即升高，然后缓慢下降；RI和PI立即下降；眼压变化后不同时间点各项指标比较，差异均有统计学意义(P＜0.01)。眼压快速升高时，大鼠虹膜血管、CRA和CRV的VD立即缩小，PSV、EDV和MV立即下降，RI和PI立即升高；眼压变化后不同时间点各项指标比较，差异均有统计学意义(P＜0.01)。结论 快速眼压波动可引起大鼠眼部血流动力学的急剧波动变化。

abstractsObjective To observe the effect of intraocular pressure(IOP)rapid fluctuations on rat ocular hemodynamics. Methods SD rat model with IOP rapid fluctuations was established. The IOP was decreased from 12 mm Hg (1 mm Hg=0. 133 kPa) to 0 mm Hg within 1 s. 2 minutes later, the IOP was increased from 0 mm Hg to 12 mm Hg within 1 s. The anterior segment photos of rats were taken by slit lamp image analysis system when IOP at 12 mm Hg and 0 mm Hg, while the iris vessels diameter (VD) was measured. The peak systolic velocity (PSV), end diastolic velocity (EDV), mean velocity (MV), resistant index (RI), pulsatility index (PI) and VD of rats central retinal artery (CRA) and central retinal vein (CRV) were detected and analyzed using color Doppler ultrasonography before and 5, 10, 20, 60s after IOP fluctuations. Results The iris VD was (28.30±5.80) or (59.80±6.40) μm when the IOP was 12 or 0 mm Hg, respectively. Compared with each other, the difference was statistically significant (t=8. 98,P＜0.01). When the IOP decreased rapidly, the VD of iris, CRA and CRV expanded immediately; PSV,EDV and MV increased immediately, then decreased slowly; RI and PI decreased immediately. The differences among different periods were statistically significant (P＜0.01). The change magnitudes of CRA were bigger than those of CRV. Conversely, when the IOP increased rapidly, the VD of iris, CRA and CRV reduced immediately; PSV, EDV and MV decreased immediately; RI and PI increased immediately. The differences among different periods were statistically significant (P ＜ 0. 01). ConclusionIOP rapid fluctuations can induce dramatic fluctuation of rat ocular hemodynamics.