摘要目的：观察硅油眼内填充后的物理性质改变。方法原发性视网膜脱离行玻璃体切割联合硅油填充手术的99例患者99只眼纳入研究。所有患眼采用23G 玻璃体切割系统行硅油取出手术,收集取出的硅油垂直静置于常温环境3 d,分层后取上层硅油。根据硅油在眼内填充的时间,将取出硅油分为1、2、3、6个月组(A、B、C、D 组),1~2年组(E 组),2年以上组(F 组),分别为12、15、25、22、13、12只眼。以未曾用于眼内填充的同型号硅油作为对照组。检测各组硅油乳化颗粒数目、运动粘度、表面张力、密度、透射率及折射系数。结果A~F 组及对照组之间硅油乳化颗粒数(F =89.337)、运动粘度(F =10.660)、表面张力(F =11.810)、密度(F =13.497)、各波长处透射率(F =455.496、566.105、525.102、767.573、622.961、601.539)比较,差异均有统计学意义(P <0.05)；折射系数比较,差异无统计学意义(F =2.936, P >0.05)。组间两两比较结果显示,与对照组比较,A 组硅油乳化颗粒数无明显改变,差异无统计学意义(P >0.05)；B~F 组硅油乳化颗粒数明显增加,差异有统计学意义(P <0.05)。A、B 组硅油运动粘度无明显改变,差异无统计学意义(P >0.05)；C~ F 组硅油运动粘度明显降低,差异有统计学意义(P <0.05)。A~D组硅油表面张力无明显改变,差异无统计学意义(P >0.05)；E、F 组硅油表面张力明显减小,差异有统计学意义(P <0.05)。A~D 组硅油密度无明显改变,差异无统计学意义(P >0.05)；E、F 组硅油密度明显增大,差异有统计学意义(P <0.05)。A~F 组硅油在各波长处的透射率明显下降,差异均有统计学意义(P <0.05)。A~F 组及对照组组内硅油在各波长处的透射率比较,差异均无统计学意义(P >0.05)。A~F 组折射系数均无明显改变,差异无统计学意义(P >0.05)。结论硅油在眼内填充2个月内乳化颗粒数增加、透射率明显降低；眼内填充3个月后,运动粘度明显降低；眼内填充1年后,表面张力明显减小、密度明显增大。

abstractsObjective To observe the changes in physical properties of silicone oil after intraocular tamponade.Methods The silicone oil was removed from 99 patients (99 eyes ) of primary retinal detachment with 23G vitreous cutter system.The upper silicone oil was collected after put the vitrectomy samples at room temperature for 3 days.According to the time of intraocular tamponade,the silicone oil samples were divide into six groups including group A (1 month,12 samples),group B (2 months,1 5 samples),group C (3 months,25 samples),group D (6 months,22 samples),group E (1 -2 years,13 samples)and group F (above 2 years,12 sample).Fresh unused silicone oil was set as blank control group. Then the emulsion particles,kinematic viscosity,surface tension,density,transmittance and refractive index were measured.Results The difference between group A-F and the control was statistical significant (P <0.05 )in emulsion particles (F = 89.337 ),kinematic viscosity (F = 10.660 ),surface tension (F =1 1.810),density (F =13.497),transmittance of wavelengths (F =455.496,566.105,525.102,767.573, 622.961,601.539),but not statistical significant at refractive index (F =2.936,P >0.05).The number of silicone oil emulsion particles has no statistical difference between group A and the control (P >0.05),but was significantly different between group B-F (P <0.05 ).The kinematic viscosity of silicone oil has no statistical difference between group A,B and the control (P >0.05),but was significantly different between group C-F (P <0.05).The surface tension of silicone oil has no statistical difference between group A-D and the control (P >0.05),but is significantly different between group E and F (P <0.05).The density of silicone oil has no statistical difference between group A - D and the control (P > 0.05 ), but was significantly different between group E and F (P <0.05).The transmittance of silicone oil has statistical difference between group A - F and the control (P < 0.05 ).The refractive index of silicone oil has no statistical difference between all the groups and the controls significantly (P > 0.05 ).Conclusions The physical properties of silicone oil will change during the intraocular tamponade.The emulsion particles number will increase and the transmittance will decrease after 2 months,the kinematic viscosity of silicone oil will decrease significantly after 3 months,and the density and surface tension will change significantly after 1 year of tamponade.