摘要目的 对hsa-miRNA-451进行靶基因预测及相关生物信息学分析.方法 选择miRBase、miRanda、TargetScan、PicTar数据库或预测软件预测hsa-miRNA-451靶基因.对预测的hsa-miRNA-451靶基因的基因集合进行功能富集分析和信号转导通路富集分析.采用Fisher精确概率法计算P值,P＜0.05为显著性阈值.结果 至少3个数据库或预测软件预测到hsa-miRNA-451靶基因的靶点18个.功能富集分析预测的结果显示,hsa-miRNA-451靶基因主要参与上皮细胞增生、发育以及调节酶活性相关生物过程(p＜0.05).信号转导通路富集分析预测的结果显示,has-miRNA-451靶基因显著富集于转化生长因子-β信号通路、丝裂原活化蛋白激酶信号通路、表皮生长因子信号通路、Wnt信号通路、哺乳动物雷帕霉素靶蛋白信号通路等(P＜0.05).结论 hsa-miRNA451可调控与上皮细胞增生、发育相关的多个信号通路.

abstractsObjective To predict as well as bioinforrmatically analyze the target genes of has-miR-451.Methods miRBase, miRanda, TargetScan and PicTar were used to predict the target genes of hsa-miRNA-451.The functions of the target genes were demonstrated by Gene Ontology and pathway enrichment analysis.P＜0.05 was set as statistically significant.Results 18 target spots of hsa-miRNA-451 were predicted by 3 databases or prediction software at least.The functions of the target genes were enriched in proliferation and development of epithelial cells and regulation of kinase activity (P＜ 0.05).Pathway analysis showed that transforming growth factor-beta signaling pathway, mitogen-activated protein kinase signaling pathway, epidermal growth factor signaling pathway, Wnt signaling pathway and mammalian target of rapamycin signaling pathway were significantly enriched (P＜0.05).Conclusion hsa-miRNA-451 might be involved in various signaling pathways related to proliferation and development of epithelial cells.