摘要目的 观察氧诱导视网膜病变(OIR)大鼠模型的全视野闪光视网膜电图(F-ERG)特征.方法 新生Sprague Dawley大鼠24只,采用简单随机的方法分为实验组和对照组.实验组大鼠建立OIR模型;于母鼠分娩完成后立即将其与新生大鼠置于氧浓度为(75±2)％的自制氧箱中.氧箱中饲养7d后再置于室内空气中饲养7d.对照组大鼠与母鼠一并置于室内空气中连续饲养14d.大鼠21日龄时,两组大鼠均行F-ERG检查,在不同的刺激光强度下记录视杆反应、最大混合反应及视锥反应.结果 与对照组比较,实验组大鼠视杆反应b波振幅降低(t=3.650)、潜伏期延长(t=2.410),差异均有统计学意义(P＜0.05);最大混合反应a、b波振幅降低(t=3.333、2.562)、潜伏期延长(t=2.725、2.482),差异均有统计学意义(P＜0.05).实验组与对照组大鼠视锥反应a、b波振幅(t=0.650、0.204)及潜伏期(t=0.422、0.076)比较,差异均无统计学意义(P＞0.05).0.001 cd.s/m2刺激光强度下大鼠F-ERG波形几乎无反应;0.010 cd.s/m2刺激光强度下大鼠F-ERG可记录到波形,随着刺激光强度的增加,b波振幅增加,a波慢慢引出.结论 OIR大鼠模型F-ERG表现为视杆反应及最大混合反应振幅降低、潜伏期延长;随着刺激光强度的增加,OIR大鼠暗适应反应b波振幅增加,a波被引出.

abstractsObjective To observe the characteristics of the full-field flash electroretinogram (F-ERG) in rats with oxygen induced retinopathy (OIR).Methods Twenty-four neonatal Sprague Dawley rats were divided into OIR group and control group.In OIR group,12 rats were exposed to (75 ± 2)％ oxygen for 7 days and then to room air for 7 days;in control group,12 rats were raised in room air for 14 days.At postnatal day 21,F-ERG tests were performed to examine the rod response,the maximum mixing reaction and the cone reaction.Results Compared with the control group,the b-wave amplitudes decreased (t=3.650) and the implicit times increased (t=2.410) in rod response in OIR group,the differences were statistically significant (P＜ 0.05);the a-and b-wave amplitudes decreased (t=3.333,2.562) and the implicit times increased (t=2.725,2.482) in the maximum mixing reaction in OIR group,the differences were statistically significant (P＜0.05).There was no difference between OIR and control group on a-and b-wave amplitudes (t=0.650,0.204) and implicit times (t=0.422,0.076) in cone response (P＞0.05).0.001 cd.s/m2 light intensity stimulation on rats F-ERG wave almost no response.0.010 cd.s/m2 light intensity stimulation on rats can be recorded to the rod response waveform,with the increase of light intensity,the amplitude of b-wave increases,the a-wave extraction.Conclusions F-ERG of OIR rat showed that the amplitude and sensitivity of the rod response and maximal rodcone response was decreased.The intensity of light had effect on the OIR rod cells,and the amplitude of b-wave increased with the increase of light intensity,the a-wave extraction.