摘要目的 研究云南省人类免疫缺陷病毒-1(HIV-1)亚型的地理及人群分布.方法 采集云南省2001-2006年1319名HIV-1感染者血液标本,分离血清后提取病毒RNA,用巢式PCR方法对gag基因片段进行扩增.扩增产物经基因序列测定,测序结果用进化树分析方法进行亚型分析.结果 成功获得了644名HIV感染者的基因序列,发现云南存在7种HIV-1亚型的流行,包括CRF08_BC、CRF07_BC、CRF07/08_BC,CRF01_AE、C、B'及URFB/C.C/CRF07_BC/CRF08_BC在各地均存在,而CRF01_AE主要存在于与缅甸接壤的德宏、保山、西双版纳和普洱.此外,还发现在感染C/CRF07_BC/CRF08_BC的人群中,61.6%(270/438)为注射吸毒感染,而感染CRF01_AE的人群中,注射吸毒传播仅占8.5%(15/177).结论 云南省存在7种HIV-1亚型流行,亚型分布与传播途径相关,并存在地域分布上的差异.

abstractsObjective To analyze the geographical distribution and risk factors of HIV-1 subtypes in Yunnan orovince.Methods Blood samples from 1319 HIV positives were collected in Yunnan Province from 2001 to 2006.The nested polymerase chain reaction was used to amplify the gag(p24)-protease fragments from RNA extracted from plasma or sera.The sequences were used for subtype determination by phylogenetic tree analysis.Results Among 1319 samples studied,the subtypes has been successfully obtained from 644 samples that were constituted of seven subtypes:CRF08_BC,CRF07_BC,CRF07/08_BC,CRFD1_AE,C,B'and URFB/C.C/CRF07_BC/CRF08_BC were distributed in the whole province,but CRF01_AE were mainly distributed in the boarding areas with Myanmar such as Dehong,Baoshan,Xishuangbanna and Puer.Moreover,injecting drugs users accounted for 61.6%(270/438)among C/CRF07_BC/CRF08_BC infections,while only 8.5%(15/177)among CRF01_AE infections.Conclusion Our data indicated that at least seven subtypes were idenfified in Yunnan province,the relationship between subtypes and transmission routes were analyzed,and the geographic difference of subtypes was also observed.