摘要目的 探讨激光诱发小鼠脉络膜新生血管(CNV)后,骨髓来源细胞(BMC)在其眼部的表型特征.方法 实验研究.以绿色荧光蛋白(GFP)转基因小鼠为供体,野生型C57BL/6J小鼠为受体进行骨髓移植后,应用532 nm倍频激光对嵌合子小鼠(处理组)和野生型小鼠(对照组)的右眼进行光凝,建立CNV模型,应用荧光素眼底血管造影和组织病理学分析确定模型稳定后,对实验眼进行免疫荧光染色,观察GFP阳性细胞在眼部的表型特征.结果 激光照射后14 d CNV生长趋于稳定,此时GFP阳性细胞分布于CNV区域(包括CNV深层的脉络膜)、CNV表层的视网膜、角巩膜缘、睫状体、视乳头周围及远离CNV的视网膜、脉络膜和巩膜.CD31或αSMA 阳性的GFP+细胞仅出现于CNV区域,F4/80和GFP双阳性的细胞不仅出现在CNV区域,还出现在CNV表层的视网膜、角巩膜缘和睫状体.结论 分化为血管细胞的BMC可特异性向CNV区域趋化,出现在眼部其他区域的BMC中有部分是巨噬细胞或树突状细胞.BMC在眼部除参与CNV形成外,可能还有其他功能.(中华眼科杂志.2008.44:212-216)

abstractsObjective To investigate the phenotype feature of bone marrow-derived cells in mice's eyes after induction of choroidal neovascularization by laser photocoagulation.Methods It was a experimental study. Green fluorescent protein(GFP)chimeric mice were developed by transplanting bone marrow cells from GFP +/+ transgenic mice to adult C57BL/6J mice.The chimeric mice underwent laser rupture of Bruch membrane to induce CNV.Fluorescein fundus angiography and histopathological study were used to confim the stable formation of CNV.Then the eyes were enucleated and processed for immunofluorescence to detect the distribution and phenotype of GFP+cells. Results The development of CNV has stabled by the 14th day after lasering. GFP-labelled cells appeared in CNV lesions(including choroid beneath CNV lesion),neurosensory retina over CNV,corneoscteral limbus,ciliary body,optic disc and sclera,retina and choroid distant from CNV.GFP+cells,which were immunoreactive for αSMA or CD31,appeared in lesions only.However,F4/80+green cells can be also detected in neurosensory retina over CNV,corneoscleral limbus and ciliary body. Condusions BMC which differentiated into vascular cells presented in CNV lesions only. Some BMC appearing in other positions might be macrophages or dendritic cells.There may be other functions apart from contributing to choroidal neovascularization for BMC in the eyes.(Chin J Ophthalmol,2008,44:212-216)