摘要目的 探讨大鼠急性视神经损伤早期即早基因c-jun、c-fos在初级视皮层的表达及其与视神经损伤程度的关系.方法 实验研究.为两因素析因设计,55只雄性SD大鼠随机入组.采用视神经钳夹和视神经离断的方法分别造成大鼠单眼急性部分视神经损伤和视神经完全性损伤的动物模型,分别于正常组和损伤后2 h、1 d、3 d、1周、1个月取脑组织行冰冻切片,以免疫荧光组织化学染色法检测损伤眼代表区初级视皮层的c-Jun、c-Fos蛋白表达并计算阳性神经元分布密度,统计学检验采用两因素析因设计计量资料的方差分析.结果 视神经钳夹组与视神经离断组的c-Jun表达差异有统计学意义(F=50.344,P=0.000).视神经钳夹组与视神经离断组的c-Jun表达差异有统计学意义(F=50.344,P=0.000).视神经损伤后2 h初级视皮层即出现c-Jun蛋白的表达升高,1 d达高峰;视神经离断组c-Jun升高幅度高于视神经钳夹组.视神经钳夹组与视神经离断组的c-Fos表达差异有统计学意义(F=62.232,P=0.000).c-Fos蛋白表达在视神经钳夹伤后2 h降低,3 d达谷底;视神经离断组降低幅度小于视神经钳夹组,且2 h时存在短暂表达升高.结论 大鼠急性视神经损伤早期初级视皮层即发生即早基因c-Jun、c-Fos的表达改变,且可能通过作用相反的途径在初级视皮层引起不同的效应.

abstractsObjective To investigate the differential expression of immediate-early genes c-jun and c-fos in primary visual cortex of rat early after acute optic nerve injury and the relationship between immediate-early gene expression and injury degree of optic nerve. Methods This was an factorial design of two factors. 55 male Sprague-Dawley rats were divided into groups randomized. Unilateral optic nerve crush injury or transection was performed in rats to obtain acute partial or complete monocular optic nerve injury models. Frozen sections through visual cortex were cut in normal rats and model rats respectively at 2 h,1 d, 3 d, 1 week and 1 month after operation. Expression of c-Jun and c-Fos was detected in primary visual cortex by means of immunohistochemistry. Statistical comparisons were made using variance analysis of factorial design. Results Statistically significant different of c-Jun expresion existed between optc nerve crash injury and transection models ( F = 50. 344, P = 0. 000 ). Increased expression of c-Jun in primary visual cortex could be observed at 2 h postoperation, and reached peak value at 1 d postoperation. The extent of increased c-Jun expression was much higher in optic nerve transection models than crush injury models. Statistically significant different of c-Fos expresion existed between optc nerve crash injury and transection models ( F = 62. 232,P = 0. 000) Decreased expression of c-Fos in primary visual cortex could be observed at 2 h postoperation in optic nerve crush injury models, and reached bottom at 3 d postoperation.The extent of decrease of c-Fos expression was lower in optic nerve transection models than crush injury models and transient increased expression could be observed at 2 h postoperation. Conclusions The expression of immediate-early genes c-Jun and c-Fos changed shortly after optic nerve injury. They may act in opposite direction in the primary visual cortex.