摘要目的 观察Nogo-A及其受体NgR在新生大鼠视觉系统发育过程中的表达及其作用.方法 实验研究.采用免疫组织化学方法对出生后1 d、3 d、7 d、14 d、28 d、56 d的SD大鼠视网膜及视皮质发育过程中Nogo-A及其受体NgR表达进行检测,并用免疫印迹法检测新生大鼠视皮质Nogo-A及NgR蛋白的表达.对所得数据进行单因素方差分析.结果 Nogo-A及其受体NgR在大鼠出生后整个发育阶段的视网膜及视皮质均可检测到阳性细胞的表达,免疫印迹法结果显示Nogo-A蛋白在1、3、7、14、28及56 d的吸光度比值分别为0.852±0.026、0.917±0.024、0.810±0.028、0.417±0.053、0.258±0.029、0.298±0.054,NgR蛋白的吸光度比值分别为0.070±0.014、0.185±0.035、0.678±0.046、0.705±0.021、1.210±0.057、1.140±0.0420,可见随着大鼠出生后的生长发育,Nogo-A蛋白表达降低(F=376.56,P=0.000),NgR蛋白表达增高(F=888.26,P=0.000).结论 Nogo-A及其受体NgR与中枢神经系统生长、发育密切相关.

abstractsObjective To study the expression of Nogo-A and Nogo receptor (NgR) in the development of neonatal rats retinal and visual cortex and their effects on the development of central nervous system (CNS). Methods Immunohistochemical staining method was used to detect the expression of NogoA and NgR in neonatal rats retinal and visual cortex at 1,3, 7, 14, 28, 56 days after birth. One-way Anova was used to analyze the results of Western blot analysis of Nogo-A and NgR in neonatal rats visual cortex.Results Nogo-A and NgR was found to express in neonatal rats retinal and visual cortex at the developing stage. In 1, 3, 7, 14, 28, 56 days, the IDV ratio of Nogo-A was 0.852 ± 0.026、0. 917 ± 0. 024、0. 810 ±0. 028、0. 417 ± 0. 053 、0. 258 ± 0. 029、0.298 ± 0.054 respectively and the IDV ratio of NgR was 0.070±0. 014、0. 185 ± 0. 035、0. 678 ± 0. 046、0. 705 ± 0. 021、1. 210 ± 0. 057、1. 140 ± 0. 0420 respectively by Western blot analysis. The expression of Nogo-A was decreased ( F = 376. 56, P = 0. 000 ) and the expression of NgR was increased ( F = 888.26, P = 0. 000) during postnatal development. Conclusion Nogo-A and NgR is closely related to the CNS growth and development.