摘要目的 为筛选出辐照灭菌中评价对病毒灭活效果的指示噬菌体,比较研究了噬菌体T4、φX174D、MS2和f2,以及两种细菌参考株大肠杆菌8099和枯草杆菌黑色变种芽孢ATCC 9372对电离辐射γ射线的抵抗力.方法 采用钴源60Co-γ射线辐射源对噬菌体和细菌进行辐照处理;灭活方法采用悬液灭活试验;噬菌体的检测采用双层琼脂法.结果 方差分析表明,不同剂量的γ射线组间(P＜0.01)和不同的噬菌体组间(P＜0.05),差异均有统计学意义.LIV(灭活对数值)和KL(杀灭对数值)为:(1)T4、ΦX174、f2和MS2 4种噬菌体经100 Gy的γ射线辐照可达到消毒水平(LIV≥4.00 log10);在此剂量下4种噬菌体的LIV分别为:6.31,6.92,5.74和4.46 log10;(2)在前述剂量下,大肠杆菌8099的KL＞7.97 log10;(3)在前述剂量下,枯草杆菌黑色变种芽孢ATCC9372的KL为1.61 log10.结论 6种微生物对γ射线的抵抗力由强到弱为:枯草杆菌黑色变种芽孢＞噬菌体MS2＞噬菌体f2＞噬菌体T4＞噬菌体φX174＞大肠杆菌.

abstractsObjective To screen the suitable bacteriophage as virus indictor in irradiation sterilization.Methods Suspensions of bacteriophage T4,φX174D,MS2,and f2,Escherichia coli 8099,and Bacillus subtilis var. niger.sp.ATCC9372 were irradiated with60 Co-γ ray.The mean log10 inactivation value levels of the bacteriophage T4,ΦX174,f2,and MS2 were 6.31,6.92,5.74,and 4.46 log10 respectively,var.niger.sp.ATCC9372 was 1.61 log10.Conclusion The order of resistance of the above six microorganisms to gamma-radiation from the biggest to the smallest iS as follows:Bacillus subtilis var.niger.sp.＞bacteriophage MS2＞bacteriophage f2＞bacteriophage T4＞bacteriophage φX 174D＞E. coli.