摘要目的 了解北京地区成人散发性病毒性胃肠炎中诺如病毒、轮状病毒及肠道腺病毒感染情况和流行病学特点,为临床防治提供理论依据.方法 收集北京大学人民医院2005年至2006年成人肠道门诊散发非霍乱水样便腹泻患者粪便标本312份,用聚丙烯酰胺凝胶电泳(PAGE)进行轮状病毒核酸的检测;分别用德国R-Biopharm公司的酶免疫试剂盒进行诺如病毒和肠道腺病毒抗原的检测.结果 312份粪便标本中,轮状病毒核酸检测全部阴性;125份粪便标本中诺如病毒阳性22份,阳性检出率17.6%(22/125),其中10月份检出率最高,为32.4%(11/34);92份粪便标本中肠道腺病毒阳性3例,检出率3.3%(3/92),其中1例诺如病毒和腺病毒混合感染.结论 北京地区成人散发性病毒性胃肠炎病原以诺如病毒多见,秋季多发;轮状病毒感染率可能很低.

abstractsObjective To understand the infection and epidemiology of norovirus and rotavirus and enteral adenovirus among adult with sporadic viral gastroenteritis in Beijing and provide theoretical basis for clinical prevention and control.Methods Stool specimens were collected from all 312 sporadic outpatient among adult with non-cholera watery diarrhea in Infectious Disease Department of Peking University People's Hospital 2005-2006.PAGE were used for detection of rotavirus RNA in stool specimens:R-Biopharm RIDASCREEN norovirus and RIDASCREEN adenovirus were used for detection of norovirus and adenovirus.Resuits Rotavirus RNA was not present in all 312 stool specimens:Norovirus was present in 17.6%(22/125)and in 32.4%(11/34)in October;Adenovirus was present in 3.3%(3/92);Mixed infections of norovirus and adenovirus was present in 1 stool specimen.Conclusions Norovirus is more common etiologic agents of sporadic acute viral gastroenteritis among adult in Beijing.The infection peak of norovirus is in autumn.Maybe the infection of rotavirus is few.