摘要目的 探讨姜黄素对离体培养的大鼠海马神经元突触传递的影响.方法 离体培养胚胎18 d SD大鼠海马神经元,9 d后加入姜黄素(终浓度10 μmol/L),继续培养24 h,采用全细胞膜片钳技术,自由记录模式,观察姜黄素对海马神经元自发兴奋性突触后电流(sEPSC)和微小兴奋性突触后电流(mEPSC)频率和幅度的影响.结果 (1)姜黄素干预的海马神经元sEPSC频率(1.4 Hz±0.5 Hz)明显高于对照组(0.8 Hz±0.4 Hz,P＜0.01);但姜黄素组海马神经元sEPSC幅度(1834 pA±244 pA)和对照组海马神经元sEPSC幅度(1721 pA±391 pA)比较差异没有统计学意义(P=0.115).(2)姜黄素干预的海马神经元mEPSC频率(6.4 Hz±0.8 Hz)明显高于对照组(5.1 Hz±0.9 Hz,P＜0.01);但姜黄素组神经元mEPSC幅度(34 pA±10 pA)和对照组海马神经元mEPSC幅度(30 pA±9pA)比较差异没有统计学意SL(P=0.057).结论 姜黄素可以增强海马神经元突触传递,这可以为最近研究发现姜黄素具有缓解脑缺血、改善AD大鼠认知功能提供神经电生理学依据.

abstractsObjective To explore the effects of curcumin on the synaptic transmission of the cultured hippocampal neurons of rats.Methods Hippocampal neurons were obtained from 18-day-old fetal SD rats,cultured for 9 days,and then divided into 2 groups:curcumin group,with curcumln of the final concentration of 10 μmol/L added into the culture fluid for 24 h,and control group with DMSO added into the cultured fluid.Simultaneous excitatory postsynaptic current(sEPSC)and miniature excitatory postsynaptic current(mEPSC)of the rat hippocampal neurons in these two groups were observed usmg the method of patch clamp,whole cell recording,and gap free mode. Results (1)The frequency of sEPSC in the neurons treated with curcumin was 1.4 Hz±0.5 Hz,significantly higher than that of the control group (0.8 Hz±0.4 Hz,P＜0.01).The amplitude of sEPSC of the neurons treated with curcumin was 1834 pA±244 pA,not significantly different from that of the control group(1721 pA±391 pA,P=0.115).(2)The frequency of mEPSC in the neurons treated with curcumin was 6.4 Hz±0.8 Hz,significantly higher than that of the control group(5.1 Hz±0.9 Hz,P＜0.001).The amplitude of mEPSC in the neurons treated with curcumin was 34 pA±10 pA.Not significantly different from that in the neurons treated with DMSO(30 pA±9 pA,P=0.057).Conclusion Curcumin modulates or enhances the synaptic transmission,which possibly contributes to the cognition enhancing effect of curcumin in rats suffering from cerebral ischemia.