摘要目的 探讨呼吸道合胞病毒感染对体外培养的人胚肺成纤维细胞细胞间黏附分子1(ICAM-1)mRNA及其蛋白表达的影响.方法 以呼吸道合胞病毒A亚型Long株攻击体外培养的人胚肺成纤维细胞,另设正常细胞组.采用实时PCR及流式细胞仪检测两组细胞ICAM-1 mRNA及其蛋白表达.结果 (1)正常细胞组24 h ICAM-1 mRNA表达为1,病毒组为2.51(P<0.05).(2)病毒组12、24、48、72和96 h后ICAM-1蛋白表达均高于正常细胞组[1.25±0.09比0.21±0.06,1.87±0.18比0.30±0.06,4.78±0.52比0.29±0.07,13.34±0.64比0.35±0.17,1.58±0.37比0.35±0.14,均P<0.01].病毒组在48～72 h维持较长时间的表达,72 h达峰值,96 h明显下降.结论 肺成纤维细胞和ICAM-1可能参与呼吸道合胞病毒肺炎的发病机制.

abstractsObjective To explore the effects of respiratory syncytial virus (RSV) upon the expressions of mRNA and protein of intercellular adhesion molecule-1 (ICAM-1) in human embryonic lung fibroblast cells. Methods The expressions of mRNA and protein of ICAM-1 were determined in human embryonic lung fibroblast cells after being infected by the LONG strain type A RSV and in normal fibroblast cells with real-time PCR and flow cytometry. Results The mRNA of ICAM-1 expression in human embryonic lung fibroblast cells was 2.51 times at 24 h post-infection as that in normal fibroblast cells(P< 0.05). The protein of ICAM-1 expression of RSV control group(1.25±0.09, 1.87±0.18, 4.78±0. 52, 13.34±0.64, 1.58±0.37) were significantly higher than those of normal cell group(0.21±0.06,0.30± 0.06,0.29±0.07,0.35±0.17,0.35±0.14)at 12, 24, 48, 72 and 96 h post-infection(all P<0.01). The protein of ICAM-1 expression of RSV control group achieved a peak value between 48 h and 72 h, and then it decreased significantly at 96 h. Conclusion Lung fibroblast cell and ICAM-1 may play some roles in pathogenic mechanism of RSV viral pneumonia.