摘要目的 研究姜黄素对卵巢癌耐紫杉醇细胞SKOV3-TR30的耐药性的逆转作用及可能机制.方法 应用噻唑蓝比色法及流式细胞检测姜黄素和(或)紫杉醇作用后SKOV3-TR30细胞增殖及细胞周期的改变;Western印迹法检测GSK-3蛋白表达变化.结果 姜黄素显著降低了紫杉醇对SKOV3 -TR30细胞的IC50,逆转倍数达3.0倍;细胞周期分析显示,只有(27.0±2.9)％的SKOV3 -TR30细胞被紫杉醇阻滞在G2-M期,而(78.5±6.4)％的SKOV3 -TR30细胞被姜黄素联合紫杉醇阻滞在G2-M期;姜黄素作用12、24h后SKOV3 -TR30中GSK-3蛋白表达水平显著下调.结论 低细胞毒剂量姜黄素可部分逆转SKOV3 -TR30细胞对紫杉醇的耐药性,下调GSK-3蛋白表达可能是其发挥逆转作用的机制之一.

abstractsObjective To explore the effects of curcumin on paclitaxel resistance reversal of SKOV3-TR30 cell line and its mechanism.Methods The ( 3-( 4,5-dimethylthiazol-2-yl )-2,5-diphenyltetrazolium bromide) MTT assay was performed to determine the sensitivity of curcumin-treated SKOV3-TR30 cells to paclitaxel.The cell cycle distribution of SKOV3-TR30 was analyzed by flow cytometry.And the expression level of glycogen synthase kinase-3 ( GSK-3 ) protein was detected by Western blot.Results IC50 of paclitaxel in SKOV3-TR30 decreased with a treatment of curcumin.And the reversal times was 3.0.Flow cytometric analysis of curcumin-treated SKOV3-TR30 cells demonstrated a distinct G2-M phase block ( 78.5 ± 6.4) ％ after a 12-hour treatment of paclitaxel versus SKOV3-TR30 cells without curcumin.There was a lack of G2-M phase arrest ( only 27.0％ ± 2.9％ ).The expression of GSK-3 protein in SKOV3-TR30 cells decreased with the 12 and 24-hour treatments of curcumin.Conclusion Curcumin can partially reverse the paclitaxel-resistance of SKOV3-TR30 cells through a down-regulation of GSK-3.