摘要目的 探讨信号素分子4C(Sema4C)在食管癌、胃癌以及直肠癌组织中的表达及其与肿瘤恶性程度的关系.方法 选择武汉同济医院病理科2008年1月至2010年12月存档的石蜡组织标本235份,其中食管癌50例,胃癌75例,直肠癌50例,以及癌旁正常黏膜各20例.应用免疫组织化学SP法检测Sema4C在各标本中的表达,并结合临床资料进行统计分析,评价Sema4C的表达情况与食管癌,胃癌及直肠癌临床病理特征的关系.结果 Sema4C在食管癌、胃癌及直肠癌组织中的阳性表达率均显著高于相应癌旁正常黏膜组织[80.0％(40例)比20.0％(4例),77.3％(58例)比25.0％(5例),80.0％(40例)比15.0％(3例),均P=0.000]；Sema4C在有淋巴结转移的癌组织中的表达阳性率均显著高于无淋巴结转移癌组织[90.3％(28例)比63.2％(12例)、85.0％(51例)比46.7％(7例)、92.0％(23例)比68.0％(17例),P=0.049、0.005、0.034].但Sema4C的表达与患者性别、年龄、肿瘤发生部位、癌细胞类型、肿瘤细胞分化程度、肿瘤大小、浸润深度及临床分期均无关(均P ＞0.05).结论 Sema4C在食管癌,胃癌以及直肠癌组织中高表达,并与淋巴结转移密切相关,可能在3种恶性肿瘤的侵袭转移过程中发挥重要作用.

abstractsObjective To explore the expression and clinical significance of signal protein Sema4C in esophageal cancer,gastric cancer and rectal cancer.Methods Fifty esophageal cancer,75 gastric cancer,50 rectal cancer and 20 corresponding normal mucous membrane specimens,collected during the period of January 2008 to December 2010,were detected with streptavidin-perosidase immunohistochemistry to detect the expression levels of Sema4C And the relationships of the Sema4C expression with clinicopathological data was analyzed.Results The expression levels of Sema4C in three kinds of cancers were significantly higher than the corresponding normal mucous membranes ( 80.0％ ( n =40 ) vs 20.0％ (n=4),77.3％ (n=58) vs25.0％ (n=5),80.0％ (n=40) vs15.0％ (n=3),allP=0.000).Furthermore,the percentage of Sema4C positive cells was significantly higher in carcinoma nests of tumors with lymphatic metastasis than those without (90.3％ ( n =28 ) vs 63.2％ ( n =12),85.0％ ( n =51 ) vs 46.7％ (n=7),92.0％ (n =23) vs 68.0％ (n=17),P=0.049,0.005,0.034).However,no significant correlations were found between the Sema4C expression with gender,age,location of tumors,types of cancer cells,cell differentiation,tumor size,depth of invasion or tumor stage (all P ＞ 0.05 ).Conclusion There is a high expression of Sema4C in esophageal cancer,gastric cancer and rectal cancer.And it is strongly correlated with lymphatic metastasis.Thus Sema4C may play critical roles in the invasion and lymphatic metastasis of esophageal cancer,gastric cancer and rectal cancer.