摘要目的 探讨腺病毒介导多巴胺2型受体短链亚型基因(D2S)联合溴隐亭药物干预对体外培养的原代人垂体无功能腺瘤(NFPA)细胞凋亡的影响.方法 应用重组腺病毒载体pAd-D2S-EGFP将D2S基因导入原代贴壁培养的人NFPA细胞中,免疫荧光染色检测感染后细胞内D2S蛋白表达变化,转染后联合溴隐亭药物干预,并以CCK-8法检测加药后细胞生长活性,并用Hoechst染色细胞核凋亡形态学检测评估转染后药物干预对细胞活性的影响.结果 pAd-D2S-EGFP能够成功感染体外贴壁培养的原代人NFPA细胞,转染D2S-EGFP联合溴隐亭药物干预能够抑制NFPA细胞生长,溴隐亭干预后第48小时时,实验组细胞活性为(40±5)％,与单纯加药组(97±5)％及空载体组(90±9)％相比,差异有统计学意义(P＜0.05).实验组Hoechst染色观察到明显的凋亡现象.结论 提高体外培养NFPA细胞的D2S表达会增加肿瘤细胞对溴隐亭治疗的敏感性.

abstractsObjective To explore the inhibitory effect of non-functioning pituitar adenoma (NFPA)cells after a combined treatment of adenovirus mediated D2S gene and bromocriptine in vitro.Methods Adenovirus containing dopamine 2 receptor short isoform (D2S) gene was used to infect NFPA cells.The transfection of D2S gene into NFPA cells was confirmed by immunofluorescence.And cell apoptosis of infected cells treated by bromocriptine was evaluated with CCK-8 assay in vitro.Results When D2S gene transfection and bromocriptine was used in combination,the survival rate of NFPA cells significantly decreased (40 ± 5)％ versus the control group (97 ± 5)％ and the pAd-EGFP transfection combined bromocriptine treatment group (90 ± 9) ％ (P ＜ 0.05).Conclusion The combined treatment of adenovirus-mediated D2S gene and bromocriptine can effectively induce the apoptosis of NFPA cells on primary culture and increase the sensitivity of NFPA to dopamine agonist.