摘要目的 观察神经干细胞(NSC)联合自组装IKVAV纳米纤维凝胶移植促进大鼠脊髓损伤的修复.方法 SD大鼠230只,随机分为凝胶治疗组、NSC治疗组、NSC联合自组装IKVAV纳米纤维凝胶移植治疗组、生理盐水治疗组和假手术组.分别于术后第1、3、5、7、14、28、56、92天进行BBB运动功能评分、免疫荧光及免疫印迹观察各组脊髓的修复情况.结果 不同治疗方式其BBB评分结果差异有统计学意义(P＜0.01).联合治疗组第3天BBB评分为(1.6±0.6)分,优于其他各治疗组,且差异有统计学意义(P＜0.001).联合移植组大鼠损伤脊髓GFAP表达低于其余各组(除假手术组),NF-200表达均高于其他治疗组(除假手术组).联合移植组BDNF和NGF的表达较其他治疗明显,差异有统计学意义(P＜0.01).结论 IKVAV纳米纤维凝胶、NSC及NSC联合自组装IKVAV纳米纤维凝胶移植均有促进损伤脊髓的功能恢复,NSC联合自组装IKVAV纳米纤维凝胶移植更有促进损伤脊髓功能的恢复的作用.

abstractsObjective To observe the effects of neural stem cells (NSC) plus self-assembly isoleucine-lysine-valine-alanine-valine (IKVAV) nanofiber gel transplantation on the promotion of function recovery of spinal cord injury (SCI) in rats.Methods A total of 230 SD rats were randomized into gel,NSC,NSC plus self-assembly IKVAV nanofiber gel transplantation,normal saline and sham-operation groups.Function repair was evaluated by bundle branch block (BBB) score,immunofluorescence and Western blot respectively at Day 1,3,5,7,14,28,56 and 92 post-operation.Results There were statistically significant differences among bundle branch block (BBB) scores of different treatment groups (P ＜ 0.01).Moreover,statistical significance existed between each treatment group and combined transplantation group (P =0.000).The expression of glial fibrillary acidic protein in combined transplantation group (rats with spinal injury) was lower than that in other treatment groups (except for sham operation) and the expression of NF-200 in this group was higher than that in other treatment groups (except for sham operation).Significant differences existed in the expressions of brain-derived neurotrophic factor and nerve growth factor between combined transplantation and other treatment groups (P ＜ 0.01).Conclusion Transplantation with IKVAV nanofiber gel,NSC and NSC plus self-assembly IKVAV nanofiber gel may promote the repair of SCI in rats.But the method of NSC plus self-assembly IKVAV nanofiber gel is more effective.