摘要目的 探讨蛋白磷酸酶2A的癌性抑制因子(CIP2A)在膀胱癌患者肿瘤组织和血清中的表达及其在膀胱癌发生发展中的作用.方法 对2010年5月至2012年12月山东大学第二医院泌尿外科收治的膀胱癌患者,采用反转录PCR (RT-PCR)检测38例患者的肿瘤组织和12例患者癌旁组织CIP2A mRNA的表达;免疫组化染色法检测99例患者肿瘤组织和12例患者癌旁组织中CIP2A蛋白表达;以ELISA法检测38例膀胱癌患者及40名健康对照者外周血中CIP2A蛋白的含量.结果 RT-PCR显示38例膀胱癌患者中29例(76.32％)检测到CIP2A mRNA的表达,癌旁组织中未有表达(P＜0.05).免疫组化染色结果显示99例膀胱癌患者中有63例(63.64％)表达了CIP2A蛋白,而癌旁组织中未有表达(P＜0.05).ELISA检测结果显示,CIP2A在膀胱癌患者外周血中的含量明显高于健康人群(中位数:0.015 2比0.001 8 ng/L,P＜0.05).结论 CIP2A在膀胱癌患者的组织及血清中高表达,CIP2A可以作为反映膀胱癌生物学行为的有效指标.

abstractsObjective To explore the expression of cancerous inhibitor of PP2A (CIP2A) and evaluate its role in bladder cancer.Methods RT-PCR was used to detect the expression of CIP2A mRNA from 38 cases of patients with bladder cancer and 12 cases of normal bladder tissue.The CIP2A protein expression levels in 99 cases of patients with bladder cancer and 12 cases of normal tissue was detected by immunohistochemical staining.And the serum contents of CIP2A protein of 38 patients with bladder cancer and 40 normal controls were detected by ELISA.Results The expression of CIP2A mRNA was detected in 29/38 cases (76.32％) of bladder cancer.And there was no expression in normal tissue (P ＜0.05).The positive rate of CIP2A protein was 63.64％ in 99 cases of bladder cancer tissues and no expression detected in normal tissues(P ＜ 0.05).ELISA results showed that the serum content of CIP2A in patients with bladder cancer was significantly higher than that in normal controls(median:0.015 2 vs 0.001 8 ng/L,P ＜ 0.05).Conclusions The tissue and serum expressions of CIP2A in patients with bladder cancer are higher than those in normal controls.And CIP2A may be used as an indicator of the biological behavior of bladder cancer.