摘要目的 研究中国口岸入境外籍人群丙型肝炎病毒(HCV)基因型及基因亚型的分布,探讨中国丙肝病毒入侵型可能的传播路径,发现未来可能传人中国的造成流行的新亚型.方法 收集2009至2013年在四川、辽宁、广东、深圳和云南等国际旅行卫生保健中心健康体检中酶联免疫法(ELISA法)筛查出的235例抗-HCV阳性的入境外籍人员的血清,提取核酸后进行荧光定量PCR测定丙肝病毒载量.以核酸(HCV-RNA)阳性样本为模板,采用逆转录半巢式PCR扩增丙肝病毒Core区基因组并进行测序,获得HCV的基因分型结果.结果 235例丙肝抗体阳性样本中检出HCV-RNA阳性129例(54.9％).获得115例阳性样本的测序结果,6种基因型均检出,其中1型72例(62.6％),2型11例(9.6％),3型21例(18.3％),4型7例(6％),5型1例(0.9％),6型3例(2.6％).115例测序样本中lb型所占比例最高,人群所占比例达到47.0％,其次是3a、la.入境外籍人员中HCV4型与欧洲及埃及病毒株亲缘性高,6型与我国病毒株亲缘性高.结论 HCV 1b型在入境外籍人员中检出率最高,警惕4型可能传人并造成流行.入境HCV感染者可能作为传染源将HCV新亚型传人中国.

abstractsObjective To monitor the the genotype and subgenotype c of hepatitis C among the immigrations.To identify the new genotype and trace the transmission.Methods From 2009 to 2013,235 positive Anti-HCV samples were collected from Sichuan,Liaoning,Guangdong,Shenzhen and Yunnan ITHC.Specific PCR primers were used to amplify the HCV Core,then the PCR products were sequenced by genetic analyzer.The genotypes were identified by aligning the GenBank reference sequences and constructing the phylogenetic tree of Core.Results One hundred and twenty-nine samples showed HCV-RNA positive (54.9％)in 235 samples which were anti-HCV positive.We detected six kinds of genotypes in 115 cases of sequncing successfully,of which 72 cases of genotype 1 (G1,62.6％),followed by G3 (18.2％),G2 (9.6％),G4 (6％),G6 (2.6％) and G5 (0.9％).Genotype 1b was the most common subtype,accounting for 47％ of all infections.The phylogenetic tress indicated the HCV 4 strains in immigrations had high affinity with that in Egypt and Europe,while HCV 6 strains closed with that in China.Conclusions HCV 1 b is the advantage of popular genotype in HCV carriers.Subtype 4 may be a possible new genotype transmited into China.The immigrations may be the sources of new genotype of HCV.