摘要目的 分析多重耐药鲍曼不动杆菌(MDR-AB)定植菌与感染菌蛋白质表达差异及MDR-AB感染的分子特征.方法 收集2008年1月至2011年12月北京同仁医院临床分离的23株血流感染MDR-AB和36株鼻部、咽部及环境定植MDR-AB,应用基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)对MDR-AB血流感染菌株和定植菌株进行鉴定和聚类分析,选择关键菌株,应用双向电泳和MALDI-TOF MS分析同一患者的血流感染MDR-AB和鼻部定植MDR-AB之间的蛋白质表达差异.结果 23株血流感染MDR-AB以ST92型为主;聚类分析显示定植与感染性MDR-AB存在MALDI-TOF MS蛋白质指纹差异;同一患者的定植与感染性MDR-AB菌株存在蛋白质组差异,通过鉴定和数据库比对发现触发因子(鉴定得分为10 218分、覆盖率为44％),次黄嘌呤单核苷酸脱氢酶(鉴定得分为1 919分、覆盖率为60％),磷酸甘油酸激酶(鉴定得分为947分、覆盖率为49％)和外膜蛋白Omp38(鉴定得分为572分、覆盖率为23％)等9种重要的差异蛋白质.结论 MDR-AB定植菌株与血流感染菌株之间存在蛋白质组表达差异,这种差异可以为MDR-AB感染分子标志研究提供实验依据.

abstractsObjective To analyze the protein expression differences between colonized multidrug-resistant acinetobacter baumannii (MDR-AB) and infectious MDR-AB using comparative proteomics and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and to explore the molecular characteristics of MDR-AB infections.Methods A total of 59 MDR-AB strains including 23 of bloodstream infections MDR-AB and 36 of nasal,pharyngeal or environmental colonization MDR-AB were collected from Beijing Tongren Hospital between January 2008 and December 2011.MALDI-TOF MS cluster analysis was applied to identify the differences of protein expression between infection strains and colonization strains (including patient colonization and environmental colonization) of MDR-AB.Two-dimensional protein differences electrophoresis and MALDI-TOF-TOF MS were used to analyze the protein expression differences between bloodstream infection strain and nasal colonization strain isolated from the same patient.Results ST92 was the main multiple locus sequence typing of 23 bloodstream infections MDR-AB.Cluster analysis showed that there were differences of protein fingerprint of MADLI-TOF MS between colonized and infectious MDR-AB.Proteomic differences were found between colonization strain and infection strain which were isolated from the same patient.And 9 major different protein spots were identified.They were trigger factor (score of identification was 10 218 and coverage rate was 44％),inosine-5'-monophosphate dehydrogenase (score of identification was 1 919 and coverage rate was 60％),phosphoglycerate kinase (score of identification was 947 and coverage rate was 49％),outer membrane protein 38 (score of identification was 572 and coverage rate was 23％) and so on.Conclusion There were differences of protein expression between MDR-AB infection strains and colonization strains.And such different proteins could provide experimental evidence for molecular markers research of MDR-AB infections.