摘要目的 通过对环状RNA(circRNA)和microRNA (miRNA)表达谱芯片的检测,寻找与急性心肌梗死(AMI)相关的circRNA和miRNA以及circRNA-miRNA相互作用关系,探析AMI的发生机制.方法 2016年11月至2017年1月新乡医学院第一附属医院住院患者,根据相关标准收集AMI患者和对照组全血样本各5例,提取total RNA,运用微阵列基因芯片进行circRNA和miRNA基因表达谱分析,运用miRanda软件预测miRNA可能结合的circRNA,对差异的circRNA对应的线性mRNA转录本进行GO和Pathway富集分析注释.结果 AMI组与对照组比较,circRNA表达谱呈显著表达:(1) AMI组差异表达的circRNA 1 670个,差异表达的miRNA 13个(P＜0.05,fc≥2);(2)AMI全血circRNA-miRNA表达呈网络相关性;(3)差异表达的circRNA涉及多个通路、疾病以及功能.结论 CircRNA与miRNA相互作用参与AMI的发生发展.

abstractsObjective Using microarray technology,to research characteristic circRNA and miRNA expression profile of acute myocardial infarction (AMI),and then explore the role of these circRNA and miRNA in gene regulation.The aim is to explore the mechanism of development of AMI.Methods The patients hospitalized in the Cardiovascular Research Center of the First Affiliated Hospital of Xinxiang Medical University between November 2016 and January 2017 were included and divided into control group and AMI group according to diagnostic criteria.We collected their whole blood and extracted the total RNA,and the expression profiles of circRNA and microRNA genes in peripheral blood of AMI were analyzed by gene chip.We predicted circRNA which was possible to combine with miRNA,and drew a network diagram,and the differentially expressed circRNA was analyzed by GO and Pathway.Results There was difference in circRNA expression profile between the control group and the AMI group.The results showed:(1) a total of 1 670 circRNA had differential expressions,and in the analysis of miRNA expression,13 miRNA had differential expressions (P ＜0.05,fc≥2);(2) multiple circRNAs-miRNAs were involved in the occurrence of AMI;(3) the analysis of GO and Pathway for differentially expressed circRNAs showed that many pathways,disease and function participated in it.Conclusion CircRNA,as an important post transcriptional regulator,is closely related to the development of AMI with miRNA.