摘要目的 应用DiversiLab系统对鲍曼不动杆菌进行基因分型,并评价其可行性.方法 对2005年全国15个城市医院临床分离的58株鲍曼不动杆菌,应用以rep-PCR为原理的DiversiLab系统分型技术进行分型,并与脉冲场凝胶电泳分型和多位点序列分型技术结果进行比较;计算Simpson相关系数(Simpson's index of diversity)比较3种方法的分辨力.结果 DiversiLab系统将58株鲍曼不动杆菌分为5簇流行克隆和25个散发克隆;多位点序列分型技术将其分为35个序列型,并归为一个克隆复合体CC22(Clone Complex 22)和35个单一体(singleton);PFGE将其分成5个流行脉冲型和34个散发克隆.DiversiLab系统、MLST和PFGE的Simpson相关系数分别为0.876、0.944和0.961.结论 DiversiLab系统的操作简单、快速,可重复性好,可以作为大量菌株首选分型方法,但分辨力低于PFGE和MLST两种分型技术.

abstractsObjective To evaluate the usefulness of DiversiLab system for genotyping of Acinetobacter baumannii.Methods Fifty-eight non-duplicated clinical Acinetobacter baumannii isolated from 15 cities in China in 2005 were typed by rep-PCR-based DiversiLab system.The results were compared with those of PFGE and multilocus sequence typing. Simpson's index of diversity was used to compare the discriminatory power among DiversiLab system, PFGE and MLST.Results Fifty-eight Acinetobacter baumannii isolates were differentiated into 5 clusters and 25 unique types by DiversiLab system. MLST identified 35 distinct sequence types, which fell into one clone complex of CC22 and 35 singletons, while PFGE resolved 5 pulsotypes and 34 unique types.Simpson's diversity indices for DiversiLab system, MLST and PFGE were O.876, O.944 and 0.961, respectively.Conclusions The discriminatory power of DiversiLab system is lower than that of PFGE and MLST.But as a simple, fast and reproducible typing method, it could be used as a first-line typing tool for the analysis of a large number of isolates.