摘要恶性肿瘤严重威胁人类健康，提高恶性肿瘤的早期诊断水平，可有效降低其病死率、改善预后。肿瘤标志物的合理应用评价及高效诊断肿瘤的标志物筛选研究仍然是目前面临的重点和难点。各种技术平台特别是新一代测序技术及相关转化医学的发展与应用，肿瘤标志物研究在蛋白质、DNA和RNA水平等均取得了迅速进展。相信随着对肿瘤发生发展基础研究的不断深入，以及临床确证技术的进步，通过前瞻性多中心研究进行科学合理地评价，制定适用于我国人群不同肿瘤的肿瘤标志物诊断策略必将成为可能。（中华检验医学杂志，2014，37：641-644）

abstractsHuman health is seriously threatened by malignancies.Early diagnosis could effectively reduce tumor mortality and improve prognosis.It remains an urgent need and difficult to evaluate the clinical application and discover more efficient diagnostic markers.The rapid development of various technology platforms, in particular the next-generation sequencing technology and translational medicine , plays crucial roles in the progress of tumor markers at the level of protein , DNA and RNA.With the development of basic researches and advances in clinical validation , and based on the prospective multicenter study , the establishment of tumor diagnostic strategy on different groups will be possible.