摘要目的：建立一种通过全自动生化分析仪检测血清活性氧代谢物（ ROMs）的方法。方法方法学建立。选取北京医院2013年9至11月之间住院患者及健康体检者混合血清标本各50份进行方法学研究，选择过氧化叔丁醇（ TBHP）作为标准品，通过全自动生化分析仪参数设置及优化，建立通过全自动生化分析仪检测ROMs的方法，生物参考区间建立选取2013年11月至12月之间北京医院体检的健康非吸烟成年人130名，其中男73名，女57名，年龄30～91（68．8±12．5）岁。参考美国国家临床和实验室标准协会（ CLSI） EP15－A2、EP17－A、EP6－A、EP7－P方案对该方法的精密度、空白限、检测限、定量检测限、线性范围、干扰因素（干扰因素包括脂肪乳、溶血、抗坏血酸和胆红素）进行评估。建立健康人生物参考区间。结果该方法批内不精密度为1．5％～4．1％，总不精密度为4．4％～9．9％；空白限为0．85 mmol／L TBHP，检测限为2．7 mmol／L TBHP，定量检测限为5．9 mmol／L TBHP；线性范围为5．9～600 mmol／L （ R2＝0．995）；四种干扰物在试验浓度内（三酰甘油≤28．58 mmol／L，血红蛋白≤173 g／L，抗坏血酸≤600 mg／L，胆红素≤73．8μmol／L）引起的偏差分别为－7．6％，4．6％，－98．9％及19．1％；健康生物参考区间为61．9～88．1 mmol／L TBHP。结论新建立的ROMs检测方法具有良好的精密度、检测限和线性范围，可满足临床检测要求，乳糜血和溶血对该方法的干扰较小，抗坏血酸、胆红素对该测试存在干扰。（中华检验医学杂志，2015，38：163－167）

abstractsObjective To establish a simple, cost-effective method to measure reactive oxygen metabolites ( ROMs) using automatic biochemical analyzer.Methods Serum samples were collected from 50 healthy individuals and 50 hospitalized patientsin Beijing Hospital from September 2013 to November 2013.By setting up and optimizing parameters on automatic biochemical analyzer, a new method of measuring ROMS was established with TBHP as calibrator.130 healthy non-smokers were recruited in Beijing Hospital from November 2013 to December 2013 to establish reference interval, including 73 males and 57 females.The average age was 68.8 ±12.5.According to CLSI documents, there was the establishment of the new method including precision, limit of blank ( LoB ) , limit of detection ( LoD ) , limit of quantitation (LoQ), linearity,interference testing, and reference interval.Results The intra assay imprecision was 1. 5%-4.1% and the total imprecision was 4.4%-9.9%.LoB was 0.85 mmol/L TBHP;LoD was 2. 7mmol/L TBHP;LoQ was 5.9mmol/L TBHP.The linearity was 5.9-600 mmol/L (R2 =0.995).When triglyceride≤28.58 mmol/L, hemoglobin≤173 g/L, vitamin C≤60 mg/dl and bilirubin≤73.8 μmol/L, the deviation was -7.6%, 4.6%, -98.9%, 19.1%respectively.The normal reference interval was 61.9 -88.1 mmol/L TBHP.Conclusions The newly-established method has good performances of precision,LOD and linearity, which can meet the clinical needs.The interference of triglyceride and hemoglobin is small, while vitamin C and bilirubin have stronger interference on measurement.( Chin J Lab Med,2015,38:163-167)